| Long name: |
FITC Conjugated, p90RSK1 (Thr359 + Ser363) Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
p90RSK Thr359 + Ser363 |
| Specificity: |
This is a highly specific antibody against p90RSK Thr359 + Ser363 |
| Modification: |
Phosphorylation |
| Modification site: |
Thr359 + Ser363 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
345-375/735 |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human p90RSK1 around the phosphorylation site of Thr359/Ser363 |
| Gene ID number: |
6195 |
| Swiss Prot: |
Q15418 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
ETV1/ER81 and NR4A1/NUR77, In fibroblast, In response to insulin, In response to mitogenic stimulation (EGF and PMA), Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', Mediates cell survival by phosphorylating the pro-apoptotic proteins BAD and DAPK1 and suppressing their pro-apoptotic function, Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the preinitiation complex, Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4), Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1, and mediates cellular proliferation, and mediates phosphorylation of RPTOR, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation, is required for EGF-stimulated phosphorylation of CREB1, phosphorylates EIF4B, phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP, regulates translation through RPS6 and EIF4B phosphorylation, survival, which potently inhibits TSC2 ability to suppress mTOR signaling, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway, which results in the subsequent transcriptional activation of several immediate-early genes, Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1 |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
90 kDa ribosomal protein S6 kinase 1, 90kD, EC 2, HU 1, HU1, MAP kinase activated protein kinase 1a, MAPKAP kinase 1A, MAPKAPK1A, MGC79981, Mitogen-activated protein kinase-activated protein kinase 1A, RPS6K1 alpha, RSK, RSK 1, RSK 1 p90, RSK1, Ribosomal S6 kinase 1, Ribosomal protein S6 kinase 90kD 1, Ribosomal protein S6 kinase 90kD polypeptide 1, Ribosomal protein S6 kinase 90kDa polypeptide 1, Ribosomal protein S6 kinase alpha 1, Ribosomal protein S6 kinase polypeptide 1, Rps6ka1, S6K alpha 1, dJ590P13, dJ590P13, p90S6K, p90rsk, polypeptide 1, pp90RSK1, rps6ka, 1, 1, 1 ribosomal protein S6 kinase, 7, 11, p90 RSK1 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
p90RSK1 (Thr359 + Ser363) Conjugated |
| Short name: |
FITC Conjugated, p90RSK1 (Thr359 + Ser363) Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, p90RSK1 (Thr359 and Ser363) (Antibody to) |
| Alternative technique: |
antibodies |