| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
MYPT1 Thr853 |
| Specificity: |
MYPT1 (Thr853), This phosphorylation site is homologous to that of Thr852 in Mouse and Thr855 in Rat |
| Modification: |
Phosphorylation |
| Modification site: |
Thr853 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human around the phosphorylation site of Thr853 |
| Gene ID: |
4659 |
| Swiss Prot: |
O14974 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
As part of the PPP1C complex, Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity, Mediates binding to myosin, involved in dephosphorylation of PLK1, Key regulator of protein phosphatase 1C (PPP1C) |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
MBS M130 |
| Also known as: |
MYPT1 (Thr853) Polyclonal Antibody |
| Other name: |
Anti-MYPT1 (Thr853) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
MYPT1 (Thr853) |
| Short name: |
Anti-MYPT1 (Thr853) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-MYPT1 (Thr853) (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
7618 |
| Gene: |
PPP1R12A |
More about : PPP1R12A |
| Long gene name: |
protein phosphatase 1 regulatory subunit 12A |
| Synonyms gene: |
MYPT1 |
| Synonyms gene name: |
protein phosphatase 1, regulatory (inhibitor) subunit 12A protein phosphatase 1, regulatory subunit 12A |
| Synonyms name: |
myosin phosphatase-targeting subunit 1 myosin binding subunit |
| Locus: |
12q21, 2-q21, 31 |
| Discovery year: |
1997-12-23 |
| GenBank acession: |
D87930 |
| Entrez gene record: |
4659 |
| Pubmed identfication: |
9286714 |
| RefSeq identity: |
NM_002480 |
| Classification: |
Ankyrin repeat domain containing Protein phosphatase 1 regulatory subunits |
| Havana BLAST/BLAT: |
OTTHUMG00000170100 |