| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
MYPT1 Ser507 |
| Specificity: |
This antibody reacts specifically with MYPT1 (Ser507) |
| Modification: |
Phosphorylation |
| Modification site: |
Ser507 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human MYPT1 around the phosphorylation site of Ser507 |
| Gene ID: |
4659 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho, Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase, Rho-associated kinase (Rho-kinase), RhoA, Several transcript variants encoding different isoforms have been found for this gene, The guanosine triphosphate(GTP)-bound, The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, Thus, [provided by RefSeq], active form of RhoA (GTP, is one of the subunits of myosin phosphatase, phosphorylated MBS and consequently inactivated myosin phosphatase, which is activated by GTP, which is also called the myosin-binding subunit of myosin phosphatase, which regulates the extent of phosphorylation of MLC, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells, Myosin phosphatase target subunit 1, RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
MBS M130 |
| Also known as: |
MYPT1 (Ser507) Polyclonal Antibody |
| Other name: |
Anti-MYPT1 (Ser507) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
MYPT1 (Ser507) |
| Short name: |
Anti-MYPT1 (Ser507) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-MYPT1 (Ser507) (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
7618 |
| Gene: |
PPP1R12A |
More about : PPP1R12A |
| Long gene name: |
protein phosphatase 1 regulatory subunit 12A |
| Synonyms gene: |
MYPT1 |
| Synonyms gene name: |
protein phosphatase 1, regulatory (inhibitor) subunit 12A protein phosphatase 1, regulatory subunit 12A |
| Synonyms name: |
myosin phosphatase-targeting subunit 1 myosin binding subunit |
| Locus: |
12q21, 2-q21, 31 |
| Discovery year: |
1997-12-23 |
| GenBank acession: |
D87930 |
| Entrez gene record: |
4659 |
| Pubmed identfication: |
9286714 |
| RefSeq identity: |
NM_002480 |
| Classification: |
Ankyrin repeat domain containing Protein phosphatase 1 regulatory subunits |
| Havana BLAST/BLAT: |
OTTHUMG00000170100 |