| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
IKK alpha Tyr463 |
| Specificity: |
This antibody reacts specifically with IKK alpha (Tyr463) |
| Modification: |
Phosphorylation |
| Modification site: |
Tyr463 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human IKK alpha around the phosphorylation site of Tyr463 |
| Gene ID: |
1147 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
IKK alpha and IKK beta interact with each other and both are essential for NFkB activation, IKK alpha specifically phosphorylates IkBa, IKK is a serine protein kinase, IKKa is expressed in variety of human tissues, NFkB is associated with IkB proteins in the cell cytoplasm, NFkB mediates the expression of a great variety of genes in response to extracellular stimuli including IL1, TNF alpha, and bacterial product LPS, and the IKK complex contains alpha and beta subunits (IKK alpha and IKK beta), which inhibit NFkB activity, Nuclear factor kappa B (NFkB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
CHUK1, Conserved Helix Loop Helix Ubiquitous Kinase, Conserved helix loop ubiquitous kinase, I Kappa B Kinase 1, IKBKA, IKK 1, IKK A, IKK a kinase, IKK alpha, IKK1, IKKA, IKKA_HUMAN, IKKalpha, IkB kinase alpha subunit, IkappaB kinase, Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B Cells, Inhibitor Of Nuclear Factor Kappa B Kinase Alpha Subunit, NFKBIKA, Nuclear Factor Kappa B Inhibitor Kinase Alpha, Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells Inhibitor, Nuclear factor NF kappa B inhibitor kinase alpha, Nuclear factor NFkappaB inhibitor kinase alpha, TCF16, I Kappa B Kinase Alpha |
| Also known as: |
IKK alpha (Tyr463) Polyclonal Antibody |
| Other name: |
Anti-IKK alpha (Tyr463) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
IKK alpha (Tyr463) |
| Short name: |
Anti-IKK alpha (Tyr463) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-IKK a (Tyr463) (polyclonal) |
| Alternative technique: |
polyclonals |