| Long name: |
FITC Conjugated, IKK alpha (Tyr463) Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
IKK alpha Tyr463 |
| Specificity: |
This is a highly specific antibody against IKK alpha Tyr463 |
| Modification: |
Phosphorylation |
| Modification site: |
Tyr463 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human IKK alpha around the phosphorylation site of Tyr463 |
| Gene ID number: |
1147 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
IKK alpha and IKK beta interact with each other and both are essential for NFkB activation, IKK alpha specifically phosphorylates IkBa, IKK is a serine protein kinase, IKKa is expressed in variety of human tissues, NFkB is associated with IkB proteins in the cell cytoplasm, NFkB mediates the expression of a great variety of genes in response to extracellular stimuli including IL1, TNF alpha, and bacterial product LPS, and the IKK complex contains alpha and beta subunits (IKK alpha and IKK beta), which inhibit NFkB activity, Nuclear factor kappa B (NFkB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
CHUK1, Conserved Helix Loop Helix Ubiquitous Kinase, Conserved helix loop ubiquitous kinase, I Kappa B Kinase 1, IKBKA, IKK 1, IKK A, IKK a kinase, IKK alpha, IKK1, IKKA, IKKA_HUMAN, IKKalpha, IkB kinase alpha subunit, IkappaB kinase, Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B Cells, Inhibitor Of Nuclear Factor Kappa B Kinase Alpha Subunit, NFKBIKA, Nuclear Factor Kappa B Inhibitor Kinase Alpha, Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells Inhibitor, Nuclear factor NF kappa B inhibitor kinase alpha, Nuclear factor NFkappaB inhibitor kinase alpha, TCF16, I Kappa B Kinase Alpha |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
IKK alpha (Tyr463) Conjugated |
| Short name: |
FITC Conjugated, IKK alpha (Tyr463) Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, IKK a (Tyr463) (Antibody to) |
| Alternative technique: |
antibodies |