| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Ephrin B Tyr329 |
| Specificity: |
This antibody reacts specifically with Ephrin B (Tyr329) |
| Modification: |
Phosphorylation |
| Modification site: |
Tyr329 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human Ephrin B around the phosphorylation site of Tyr329 |
| Gene ID: |
1947 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Ephrin B molecules expressed at the membrane surface bind to the Ephrin B family receptors on target cells during cell to cell contact, Importantly, Recent work demonstrated that Tyr331 of Ephrin B was phosphorylated in HEK293 cells after stimulation by the soluble Ephrin B2 receptor tyrosine kinase, This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing Ephrin B on its surface, This reverse signaling event is thought to be critical for vessel maturation and neuronal development, tyrosine phosphorylation of Ephrin B is thought to be a critical component of this reverse signaling event, Ephrin B proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
CEK5 ligand, EFNB1, EFNB2_HUMAN, ELK L, ELK ligand, ELKL, EPH related receptor tyrosine kinase ligand 2, Ephrin B Precursor, Ephrin B1, Ephrin B2, Ephrin-B, EphrinB, LERK 2, LERK2, p-Ephrin B phospho Y329, Ephrin B phospho Y329 |
| Also known as: |
Ephrin B (Tyr329) Polyclonal Antibody |
| Other name: |
Anti-Ephrin B (Tyr329) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Ephrin B (Tyr329) |
| Short name: |
Anti-Ephrin B (Tyr329) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Ephrin B (Tyr329) (polyclonal) |
| Alternative technique: |
polyclonals |