| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
FAK Tyr397 |
| Specificity: |
FAK (Tyr397), This phosphorylation site is homologous to that of Tyr428 in Mouse and Tyr397 in Rat |
| Modification: |
Phosphorylation |
| Modification site: |
Tyr397 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cell membrane, Nucleus, Cytoplasm |
| Antigen Source: |
KLH cunjugated synthetic phosphopeptide derived from human FAK 1 around the phosphorylation site of Tyr397 |
| Gene ID: |
5747 |
| Swiss Prot: |
Q05397 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix, Dec 2010], Several transcript variants encoding different isoforms have been found for this gene, The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies, [provided by RefSeq, but the full-length natures of only three of them have been determined, This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
FAK FADK FAK1 PPP1R71 |
| Also known as: |
FAK (Tyr397) Polyclonal Antibody |
| Other name: |
Anti-FAK (Tyr397) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
FAK (Tyr397) |
| Short name: |
Anti-FAK (Tyr397) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-FAK (Tyr397) (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
9611 |
| Gene: |
PTK2 |
More about : PTK2 |
| Long gene name: |
protein tyrosine kinase 2 |
| Synonyms gene name: |
PTK2 protein tyrosine kinase 2 |
| Synonyms name: |
protein phosphatase 1, regulatory subunit 71 |
| Locus: |
8q24, 3 |
| Discovery year: |
1992-10-02 |
| GenBank acession: |
L13616 |
| Entrez gene record: |
5747 |
| Pubmed identfication: |
8422239 |
| RefSeq identity: |
NM_005607 |
| Classification: |
Protein phosphatase 1 regulatory subunits FERM domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000067438 |