| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
14-3-3 protein zeta/delta Ser58 |
| Specificity: |
14-3-3 protein zeta/delta (S58), This antibody will react with all 14-3-3 proteins containing the conserved motif RS(S-p)WR |
| Modification: |
Phosphorylation |
| Modification site: |
Ser58 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human 14-3-3 protein zeta/delta around the phosphorylation site of Ser58 [RS(p-S)WR] |
| Gene ID: |
7529, 7534, 10971 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases, Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation amoung many other functions, They are also present, and are axonally transported to the nerve terminals, at lower levels, in various other eukaryotic tissues, including those involved in cell cycle regulation and cell survival, localized in neurons, Members of the 14-3-3 family of proteins are highly conserved proteins |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
HS1 14-3-3 |
| Also known as: |
14-3-3 protein zeta/delta (S58) Antibody |
| Other name: |
Anti-14-3-3 protein zeta/delta (S58) |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Gene target: |
14-3-3 protein zeta/delta (S58) |
| Short name: |
Anti-14-3-3 protein zeta/delta (S58) Fluor 594 |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-14-3-3 protein zeta/delta (S58) |
| Identity: |
12854 |
| Gene: |
YWHAQ |
More about : YWHAQ |
| Long gene name: |
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta |
| Synonyms gene name: |
theta , theta polypeptide protein, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein |
| Synonyms name: |
protein tau |
| Locus: |
2p25, 1 |
| Discovery year: |
1999-08-20 |
| GenBank acession: |
AF070556 |
| Entrez gene record: |
10971 |
| Pubmed identfication: |
11080204 |
| RefSeq identity: |
NM_006826 |
| Classification: |
14-3-3 phospho-serine/phospho-threonine binding proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000013848 |