| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
PHGDH |
| Specificity: |
This antibody reacts specifically with PHGDH |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Intracellular |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human PHGDH |
| Gene ID: |
26227 |
| Swiss Prot: |
O43175 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
3PGDH is regulated at the transcriptional level depending on tissue specificity and cellular proliferative status (2), 3PGDH protein is 544 amino acids in length, 3PGDH protein is also highly expressed in adult and fetal brain tissues (3), 3PGDH protein plays an important role in the metabolism, The trophic actions of glial cells on Purkinje neurons are mediated by L-serine and glycine, Two distinct mRNA transcripts that encode for 3PGDH protein in normal human tissues are dominant 2, and 710 bp mRNA, and function of the central nervous system (3) and its deficiency is a treatable congential error (4-5) that impairs L-serine biosynthesis which is characterized by congenital microcephaly, and heart, and pancreas, and seizures (3), and weakly expressed in thymus, brain, colon, development, kidney, liver, ovary, psychomotor retardation, testis, which are glia-derived trophic factors synthesized by 3PGDH (1), which is highly expressed in heart and skeletal muscle (2), which is highly expressed in prostate, 1 kb mRNA, The survival and development of central neurons require the supply of trophic factors by glial cells |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
SERA PGDH PDG |
| Also known as: |
PHGDH Polyclonal Antibody |
| Other name: |
Anti-PHGDH Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
PHGDH |
| Short name: |
Anti-PHGDH (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-PHGDH (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
8923 |
| Gene: |
PHGDH |
More about : PHGDH |
| Long gene name: |
phosphoglycerate dehydrogenase |
| Locus: |
1p12 |
| Discovery year: |
1999-11-22 |
| GenBank acession: |
BC011262 |
| Entrez gene record: |
26227 |
| RefSeq identity: |
NM_006623 |
| Havana BLAST/BLAT: |
OTTHUMG00000012100 |