| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Smoothened homolog |
| Specificity: |
This antibody reacts specifically with Smoothened homolog |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Smoothened |
| Gene ID: |
6608 |
| Swiss Prot: |
Q99835 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO), Required for the accumulation of KIF7 and GLI3 in the cilia, G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
FZD11 |
| Also known as: |
Smoothened homolog Polyclonal Antibody |
| Other name: |
Anti-Smoothened homolog Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Smoothened homolog |
| Short name: |
Anti-Smoothened homolog (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Smoothened homolog (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
11119 |
| Gene: |
SMO |
More about : SMO |
| Long gene name: |
frizzled class receptor , smoothened |
| Synonyms gene: |
SMOH |
| Synonyms gene name: |
frizzled family receptor , seven transmembrane spanning receptor smoothened, smoothened (Drosophila) homolog smoothened homolog (Drosophila) smoothened |
| Synonyms name: |
frizzled family member 11 |
| Locus: |
7q32, 1 |
| Discovery year: |
1996-12-18 |
| GenBank acession: |
U84401 |
| Entrez gene record: |
6608 |
| Pubmed identfication: |
9628830 |
| RefSeq identity: |
NM_005631 |
| Classification: |
Class F frizzled , G protein-coupled receptors |
| Havana BLAST/BLAT: |
OTTHUMG00000158421 |