| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Bcl6 |
| Specificity: |
This antibody reacts specifically with Bcl6 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Bcl6 |
| Gene ID: |
604 |
| Swiss Prot: |
P41182 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Also required for the establishment and maintenance of immunological memory for both T- and B-cells, An important function is to allow GC B-cells to proliferate very rapidly in response to T-cell dependent antigens and tolerate the physiological DNA breaks required for immunglobulin class switch recombination and somatic hypermutation without inducing a p53/TP53-dependent apoptotic response, Besides, Forms complexes with different corepressors and histone deacetylases to repress the transcriptional expression of different subsets of target genes, In GC B-cells, In follicular helper CD4(+) T-cells (T(FH) cells), In response to genotoxic stress, Represses its target genes by binding directly to the DNA sequence 5'-TTCCTAGAA-3' (BCL6-binding site) or indirectly by repressing the transcriptional activity of transcription factors, Suppresses macrophage proliferation through competition with STAT5 for STAT-binding motifs binding on certain target genes, T(H)2 and T(H)17 cells, also autoregulates its transcriptional expression and up-regulates, also controls neurogenesis through the alteration of the composition of NOTCH-dependent transcriptional complexes at selective NOTCH targets, apoptosis and cell cycle control, controls cell cycle arrest in GC B-cells in both p53/TP53-dependedent and -independent manners, including the recruitment of the deacetylase SIRT1 and resulting in an epigenetic silencing leading to neuronal differentiation, indirectly, inflammation, like miR155, promotes the expression of T(FH)-related genes but inhibits the differentiation of T(H)1, represses genes that function in differentiation, such as AICDA, such as CCL2 and CCND2, such as HES5, the expression of some genes important for GC reactions, through the repression of microRNAs expression, Transcriptional repressor mainly required for germinal center (GC) formation and antibody affinity maturation which has different mechanisms of action specific to the lineage and biological functions |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
FLJ39821 |
| Also known as: |
Bcl6 Polyclonal Antibody |
| Other name: |
Anti-Bcl6 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Bcl6 |
| Short name: |
Anti-Bcl6 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-B-cell CLL/lymphoma 6 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
23953 |
| Gene: |
BCORP1 |
More about : BCORP1 |
| Long gene name: |
BCL6 corepressor pseudogene 1 |
| Synonyms gene: |
BCORL2 |
| Synonyms gene name: |
BCL6 co-repressor-like 2 BCL6 corepressor-like 2 |
| Locus: |
Yq11, 222 |
| Discovery year: |
2004-07-27 |
| GenBank acession: |
AK097140 |
| Entrez gene record: |
286554 |
| RefSeq identity: |
NM_173700 |
| Havana BLAST/BLAT: |
OTTHUMG00000036498 |