| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
FOXO1 |
| Specificity: |
This antibody reacts specifically with FOXO1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human FOXO1 |
| Gene ID: |
2308 |
| Swiss Prot: |
Q12778 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Activity suppressed by insulin, Acts synergistically with ATF4 to suppress osteocalcin/BGLAP activity, Also suppresses the transcriptional activity of RUNX2, Binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'-TT[G/A]TTTAC-3', G6PC and PCK1, Important regulator of cell death acting downstream of CDK1, In hepatocytes, Main regulator of redox balance and osteoblast numbers and controls bone mass, Mediates insulin action on adipose tissue, Orchestrates the endocrine function of the skeleton in regulating glucose metabolism, PKB/AKT1 and SKT4/MST1, Promotes neural cell death, Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, Regulates the transcriptional activity of GADD45A and repair of nitric oxide-damaged DNA in beta-cells, Required for the autophagic cell death induction in response to starvation or oxidative stress in a transcription-independent manner, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake, an upstream activator of osteocalcin/BGLAP, increasing glucose levels and triggering glucose intolerance and insulin insensitivity, promotes gluconeogenesis by acting together with PPARGC1A and CEBPA to activate the expression of genes such as IGFBP1, Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
FKH1 |
| Also known as: |
FOXO1 Polyclonal Antibody |
| Other name: |
Anti-FOXO1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
FOXO1 |
| Short name: |
Anti-FOXO1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-forkhead box O1 (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
DNA-templated and biological process this GO :0006355 and regulation of transcription, DNA-templated and biological process this GO :0006357 and regulation of transcription from RNA polymerase II promoter and biological process this GO :0006473 and protein acetylation and biological process this GO :0006915 and apoptotic process and biological process this GO :0006974 and cellular response to DNA damage stimulus and biological process this GO :0007173 and epidermal growth factor receptor signaling pathway and biological process this GO :0008286 and insulin receptor signaling pathway and biological process this GO :0008543 and fibroblast growth factor receptor signaling pathway and biological process this GO :0009267 and cellular response to starvation and biological process this GO :0010508 and positive regulation of autophagy and biological process this GO :0031018 and endocrine pancreas development and biological process this GO :0031625 and ubiquitin protein ligase binding and molecular function this GO :0032869 and cellular response to insulin stimulus and biological process this GO :0032873 and negative regulation of stress-activated MAPK cascade and biological process this GO :0034599 and cellular response to oxidative stress and biological process this GO :0035947 and regulation of gluconeogenesis by regulation of transcription from RNA polymerase II promoter and biological process this GO :0038095 and Fc-epsilon receptor signaling pathway and biological process this GO :0042127 and regulation of cell proliferation and biological process this GO :0042593 and glucose homeostasis and biological process this GO :0043065 and positive regulation of apoptotic process and biological process this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043565 and sequence-specific DNA binding and molecular function this GO :0045087 and innate immune response and biological process this GO :0045444 and fat cell differentiation and biological process this GO :0045599 and negative regulation of fat cell differentiation and biological process this GO :0045722 and positive regulation of gluconeogenesis and biological process this GO :0045732 and positive regulation of protein catabolic process and biological process this GO :0045892 and negative regulation of transcription, DNA-templated and biological process this GO :0045893 and positive regulation of transcription, DNA-templated and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0048011 and neurotrophin TRK receptor signaling pathway and biological process this GO :0048015 and phosphatidylinositol-mediated signaling and biological process this GO :0051721 and protein phosphatase 2A binding and molecular function this GO :0070417 and cellular response to cold and biological process this GO :0071455 and cellular response to hyperoxia and biological process this GO :0071732 and cellular response to nitric oxide and biological process this GO :2000505 and regulation of energy homeostasis and biological process, FKH1 and FKHR and FOXO1A, FOXO1 and IDBG-26475 and ENSG00000150907 and 2308, FOXO1 and IDBG-629639 and ENSBTAG00000044105 and 506618, Foxo1 and IDBG-143370 and ENSMUSG00000044167 and 56458, nuclei, protein phosphatase 2A binding, this GO :0000122 and negative regulation of transcription from RNA polymerase II promoter and biological process this GO :0000989 and transcription factor binding transcription factor activity and molecular function this GO :0001078 and RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription and molecular function this GO :0001568 and blood vessel development and biological process this GO :0001659 and temperature homeostasis and biological process this GO :0001678 and cellular glucose homeostasis and biological process this GO :0003677 and DNA binding and molecular function this GO :0003682 and chromatin binding and molecular function this GO :0003700 and sequence-specific DNA binding transcription factor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005739 and mitochondrion and cellular component this GO :0005829 and cytosol and cellular component this GO :0006351 and transcription, this GO :0000989 : transcription factor binding transcription factor activity, this GO :0000989 : transcription factor binding transcription factor activity and also this GO :0001078 : RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription and also this GO :0003677 : DNA binding and also this GO :0003682 : chromatin binding and also this GO :0003700 : sequence-specific DNA binding transcription factor activity and also this GO :0005515 : protein binding and also this GO :0031625 : ubiquitin protein ligase binding and also this GO :0043565 : sequence-specific DNA binding and also this GO :0051721 : protein phosphatase 2A binding, this GO :0001078 : RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription, this GO :0003677 : DNA binding, this GO :0003682 : chromatin binding, this GO :0003700 : sequence-specific DNA binding transcription factor activity, this GO :0005515 : protein binding, this GO :0031625 : ubiquitin protein ligase binding, this GO :0043565 : sequence-specific DNA binding, this GO :0051721 : protein phosphatase 2A binding, forkhead box O1 |
| Identity: |
3819 |
| Gene: |
FOXO1 |
More about : FOXO1 |
| Long gene name: |
forkhead box O1 |
| Synonyms gene: |
FKHR FOXO1A |
| Synonyms gene name: |
forkhead homolog in rhabdomyosarcoma |
| Locus: |
13q14, 11 |
| Discovery year: |
1994-01-07 |
| Entrez gene record: |
2308 |
| Pubmed identfication: |
8275086 15057823 |
| RefSeq identity: |
NM_002015 |
| Classification: |
Forkhead boxes |
| Havana BLAST/BLAT: |
OTTHUMG00000016775 |