| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
TWIST |
| Specificity: |
This antibody reacts specifically with TWIST protein |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human TWIST |
| Gene ID: |
7291 |
| Swiss Prot: |
Q15672 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Activates transcription as a heterodimer with E proteins, Also represses expression of proinflammatory cytokines such as TNFA and IL1B, Heterodimerization is also required for osteoblast differentiation, Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression, Inhibits myogenesis by sequestrating E proteins, Regulates cranial suture patterning and fusion, Regulates gene expression differentially, Represses the activity of the circadian transcriptional activator: NPAS2-ARNTL/BMAL1 heterodimer (By similarity), This interaction probably involves the basic domains of both proteins, and inhibiting DNA-binding by MYOD1 through physical interaction, depending on dimer composition, inhibiting trans-activation by MEF2, Acts as a transcriptional regulator |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
SCS H-twist BPES2 bHLHa38 CRS1 |
| Also known as: |
TWIST protein Polyclonal Antibody |
| Other name: |
Anti-TWIST protein Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
TWIST protein |
| Short name: |
Anti-TWIST protein (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-TWIST protein (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
12428 |
| Gene: |
TWIST1 |
More about : TWIST1 |
| Long gene name: |
twist family bHLH transcription factor 1 |
| Synonyms gene: |
ACS3 BPES3 TWIST CRS |
| Synonyms gene name: |
blepharophimosis, epicanthus inversus and ptosis 3 acrocephalosyndactyly 3 twist homolog 1 (Drosophila) twist basic helix-loop-helix transcription factor 1 craniosynostosis |
| Synonyms name: |
Saethre-Chotzen syndrome |
| Locus: |
7p21, 1 |
| Discovery year: |
1997-07-01 |
| GenBank acession: |
U80998 |
| Entrez gene record: |
7291 |
| Pubmed identfication: |
8995765 11474656 17343269 |
| RefSeq identity: |
NM_000474 |
| Classification: |
Basic helix-loop-helix proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000090821 |