| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
FOXA2/HNF 3beta |
| Specificity: |
This antibody reacts specifically with FOXA2/HNF 3beta |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human HNF 3 beta |
| Gene ID: |
3170 |
| Swiss Prot: |
Q9Y261 |
| Applications: |
IF(IHC-P), FCM |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200), FCM(1:20-100) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity), Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation, FOXA1 and FOXA2 seem to have at least in part redundant roles, In embryonic development is required for notochord formation, Interacts with the cis-acting regulatory regions of these genes, Involved in glucose homeostasis, Involved in regulation of fat metabolism, Involved in the development of multiple endoderm-derived organ systems such as the liver, Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites, Originally described as a transcription activator for a number of liver genes such as AFP, PEPCK, albumin, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues, etc, pancreas and lungs, regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis, tyrosine aminotransferase, Transcription factor that is involved in embryonic development |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
FOXA2/HNF 3beta Polyclonal Antibody |
| Other name: |
Anti-FOXA2/HNF 3beta Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
FOXA2/HNF 3beta |
| Short name: |
Anti-FOXA2/HNF 3beta (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-forkhead box A2/HNF 3beta (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
DNA-templated and biological process this GO :0006357 and regulation of transcription from RNA polymerase II promoter and biological process this GO :0006366 and transcription from RNA polymerase II promoter and biological process this GO :0007389 and pattern specification process and biological process this GO :0008134 and transcription factor binding and molecular function this GO :0008344 and adult locomotory behavior and biological process this GO :0009653 and anatomical structure morphogenesis and biological process this GO :0009952 and anterior/posterior pattern specification and biological process this GO :0009953 and dorsal/ventral pattern formation and biological process this GO :0010468 and regulation of gene expression and biological process this GO :0010719 and negative regulation of epithelial to mesenchymal transition and biological process this GO :0016568 and chromatin modification and biological process this GO :0019216 and regulation of lipid metabolic process and biological process this GO :0019218 and regulation of steroid metabolic process and biological process this GO :0019904 and protein domain specific binding and molecular function this GO :0021533 and cell differentiation in hindbrain and biological process this GO :0021904 and dorsal/ventral neural tube patterning and biological process this GO :0023019 and signal transduction involved in regulation of gene expression and biological process this GO :0030193 and regulation of blood coagulation and biological process this GO :0030324 and lung development and biological process this GO :0031018 and endocrine pancreas development and biological process this GO :0032525 and somite rostral/caudal axis specification and biological process this GO :0033132 and negative regulation of glucokinase activity and biological process this GO :0040019 and positive regulation of embryonic development and biological process this GO :0043433 and negative regulation of sequence-specific DNA binding transcription factor activity and biological process this GO :0043565 and sequence-specific DNA binding and molecular function this GO :0044212 and transcription regulatory region DNA binding and molecular function this GO :0045087 and innate immune response and biological process this GO :0045665 and negative regulation of neuron differentiation and biological process this GO :0045666 and positive regulation of neuron differentiation and biological process this GO :0045880 and positive regulation of smoothened signaling pathway and biological process this GO :0045893 and positive regulation of transcription, DNA-templated and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0045945 and positive regulation of transcription from RNA polymerase III promoter and biological process this GO :0048468 and cell development and biological process this GO :0048646 and anatomical structure formation involved in morphogenesis and biological process this GO :0048665 and neuron fate specification and biological process this GO :0060441 and epithelial tube branching involved in lung morphogenesis and biological process this GO :0060487 and lung epithelial cell differentiation and biological process this GO :0061178 and regulation of insulin secretion involved in cellular response to glucose stimulus and biological process this GO :0061448 and connective tissue development and biological process this GO :0070741 and response to interleukin-6 and biological process this GO :0071542 and dopaminergic neuron differentiation and biological process this GO :0090009 and primitive streak formation and biological process this GO :2000049 and positive regulation of cell-cell adhesion mediated by cadherin and biological process this GO :2000543 and positive regulation of gastrulation and biological process this GO :2000971 and negative regulation of detection of glucose and biological process this GO :2000976 and regulation of transcription from RNA polymerase II promoter involved in detection of glucose and biological process, FOXA2 and IDBG-59234 and ENSG00000125798 and 3170, FOXA2 and IDBG-648756 and ENSBTAG00000012407 and 503580, Foxa2 and IDBG-208945 and ENSMUSG00000037025 and 15376, HNF3B and TCF3B, nuclei, this GO :0000432 and positive regulation of transcription from RNA polymerase II promoter by glucose and biological process this GO :0000433 and negative regulation of transcription from RNA polymerase II promoter by glucose and biological process this GO :0000978 and RNA polymerase II core promoter proximal region sequence-specific DNA binding and molecular function this GO :0001205 and RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription and molecular function this GO :0001701 and in utero embryonic development and biological process this GO :0001705 and ectoderm formation and biological process this GO :0001708 and cell fate specification and biological process this GO :0003677 and DNA binding and molecular function this GO :0003700 and sequence-specific DNA binding transcription factor activity and molecular function this GO :0003705 and RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0006355 and regulation of transcription, this GO :0000978 : RNA polymerase II core promoter proximal region sequence-specific DNA binding, this GO :0000978 : RNA polymerase II core promoter proximal region sequence-specific DNA binding and also this GO :0001205 : RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription and also this GO :0003677 : DNA binding and also this GO :0003700 : sequence-specific DNA binding transcription factor activity and also this GO :0003705 : RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity and also this GO :0005515 : protein binding and also this GO :0008134 : transcription factor binding and also this GO :0019904 : protein domain specific binding and also this GO :0043565 : sequence-specific DNA binding and also this GO :0044212 : transcription regulatory region DNA binding, this GO :0001205 : RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription, this GO :0003677 : DNA binding, this GO :0003700 : sequence-specific DNA binding transcription factor activity, this GO :0003705 : RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity, this GO :0005515 : protein binding, this GO :0008134 : transcription factor binding, this GO :0019904 : protein domain specific binding, this GO :0043565 : sequence-specific DNA binding, this GO :0044212 : transcription regulatory region DNA binding, transcription regulatory region DNA binding, forkhead box A2 |
| Identity: |
5022 |
| Gene: |
FOXA2 |
More about : FOXA2 |
| Long gene name: |
forkhead box A2 |
| Synonyms gene: |
HNF3B |
| Synonyms gene name: |
beta , hepatocyte nuclear factor 3 |
| Locus: |
20p11, 21 |
| Discovery year: |
1998-02-11 |
| GenBank acession: |
AF147787 |
| Entrez gene record: |
3170 |
| Pubmed identfication: |
9119385 11875061 |
| Classification: |
Forkhead boxes |
| Havana BLAST/BLAT: |
OTTHUMG00000191876 |