| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
PARK2 |
| Specificity: |
This antibody reacts specifically with Parkin/PARK2 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human E3 ubiquitin-protein ligase parkin |
| Gene ID: |
5071 |
| Swiss Prot: |
O60260 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
CCNE1, GPR37, GPR37 accumulation, In collaboration with CHPF isoform 2, Independently of its ubiquitin ligase activity, Limits the production of reactive oxygen species (ROS), MFN1, MFN2, May play a role in controlling neurotransmitter trafficking at the presynaptic terminal and in calcium-dependent exocytosis, May protect neurons against alpha synuclein toxicity, May represent a tumor suppressor gene, Mediates 'Lys-48'-linked polyubiquitination of ZNF746, Mediates 'Lys-63'-linked polyubiquitination of SNCAIP, Mediates monoubiquitination as well as 'Lys-48'-linked and 'Lys-63'-linked polyubiquitination of substrates depending on the context, Mediates monoubiquitination of BCL2, Participates in the removal and/or detoxification of abnormally folded or damaged protein by mediating 'Lys-63'-linked polyubiquitination of misfolded proteins such as PARK7: 'Lys-63'-linked polyubiquitinated misfolded proteins are then recognized by HDAC6, Promotes the autophagic degradation of dysfunctional depolarized mitochondria (mitophagy) by the ubiquitination of mitochondrial proteins such as TOMM2, RHOT1/MIRO1, RHOT1/MIRO1 and USP3 (PubMed:24896179), Regulates cyclin-E during neuronal apoptosis, SEPT5, STUB1, SYT11, TOMM2, USP3, ZNF746 and AIMP2, a 22 kDa O-linked glycosylated isoform of SNCAIP, and kainate-induced excitotoxicity, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins, followed by degradation, followed by degradation of ZNF746 by the proteasome, leading to their recruitment to aggresomes, may enhance cell viability and protect cells from oxidative stress, possibly playing a role in Lewy-body formation, possibly playing a role in the regulation of neuron death, proteasomal dysfunction, protects from apoptosis by the transcriptional repression of p53/TP53, such as BCL2, thereby acting as a positive regulator of autophagy, Functions within a multiprotein E3 ubiquitin ligase complex |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
PDJ AR-JP parkin PRKN |
| Also known as: |
Parkin/PARK2 Polyclonal Antibody |
| Other name: |
Anti-Parkin/PARK2 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Parkin/PARK2 |
| Short name: |
Anti-Parkin/PARK2 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Parkin/PARK2 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
8607 |
| Gene: |
PARK2 |
More about : PARK2 |
| Long gene name: |
parkin RBR E3 ubiquitin protein ligase |
| Synonyms gene name: |
E3 ubiquitin protein ligase (parkin) , Parkinson disease (autosomal recessive, juvenile) 2, parkin parkinson protein 2 |
| Synonyms name: |
E3 ubiquitin ligase |
| Locus: |
6q26 |
| Discovery year: |
1997-08-18 |
| Entrez gene record: |
5071 |
| Pubmed identfication: |
9560156 9570960 |
| Classification: |
Parkinson disease associated genes RBR E3 ubiquitin ligases |
| Havana BLAST/BLAT: |
OTTHUMG00000015970 |
| Locus Specific Databases: |
Parkinson', s disease Mutation Database |