| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
HSP20 |
| Specificity: |
This antibody reacts specifically with HSP20 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human HSP20 |
| Gene ID: |
126393 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Hsp20 is diffusely distributed in the cytosol, Hsp20 may form different heterocomplexes with other Hsp's, Hsp27 and may form different hetercomplexes with these proteins, In normal conditions Hsp20 is diffusely disturbed in cytosol but under the heat stress it undergoes translocation to membrane fraction, It is distributed ubiquitously in tissues, Phosphorylated form of Hsp20 is proposed to interact with monomeric actin whereas dephosphorylated form binds polymeric actin filaments, The Hsp20 was demonstrated to constitute up to 1, The specific physiological function of Hsp20 is not yet known, Under normal conditions, Unlike other heat shock proteins the amount of Hsp20 does not increase after heat shock, and its expression is related to muscle contraction, but is found in higher levels in skeletal, but under heat stress conditions, especially in muscle, it translocates to the nucleus, smooth and heart muscle, specifically in slow-twitch muscle, such as alpha-crystalline and Hsp25, 3% of the total cellular protein in vertebrate tissues, Hsp20 is a small heat shock protein related to Hsp25 |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
FLJ32389 Hsp20 PPP1R91 |
| Also known as: |
HSP20 Polyclonal Antibody |
| Other name: |
Anti-HSP20 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
HSP20 |
| Short name: |
Anti-HSP20 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-HSP20 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
26511 |
| Gene: |
HSPB6 |
More about : HSPB6 |
| Long gene name: |
heat shock protein family B (small) member 6 |
| Synonyms gene name: |
B6 , alpha-crystallin-related, heat shock protein |
| Synonyms name: |
protein phosphatase 1, regulatory subunit 91 |
| Locus: |
19q13, 12 |
| Discovery year: |
2004-05-12 |
| GenBank acession: |
AJ278121 |
| Entrez gene record: |
126393 |
| Pubmed identfication: |
12820654 14717697 |
| RefSeq identity: |
NM_144617 |
| Classification: |
Small heat shock proteins Protein phosphatase 1 regulatory subunits |
| Havana BLAST/BLAT: |
OTTHUMG00000048122 |