| Long name: |
FITC Conjugated, HSP20 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
HSP20 |
| Specificity: |
This is a highly specific antibody against HSP20 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human HSP20 |
| Gene ID number: |
126393 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Hsp20 is diffusely distributed in the cytosol, Hsp20 may form different heterocomplexes with other Hsp's, Hsp27 and may form different hetercomplexes with these proteins, In normal conditions Hsp20 is diffusely disturbed in cytosol but under the heat stress it undergoes translocation to membrane fraction, It is distributed ubiquitously in tissues, Phosphorylated form of Hsp20 is proposed to interact with monomeric actin whereas dephosphorylated form binds polymeric actin filaments, The Hsp20 was demonstrated to constitute up to 1, The specific physiological function of Hsp20 is not yet known, Under normal conditions, Unlike other heat shock proteins the amount of Hsp20 does not increase after heat shock, and its expression is related to muscle contraction, but is found in higher levels in skeletal, but under heat stress conditions, especially in muscle, it translocates to the nucleus, smooth and heart muscle, specifically in slow-twitch muscle, such as alpha-crystalline and Hsp25, 3% of the total cellular protein in vertebrate tissues, Hsp20 is a small heat shock protein related to Hsp25 |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
FLJ32389 Hsp20 PPP1R91 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
HSP20 Conjugated |
| Short name: |
FITC Conjugated, HSP20 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, HSP20 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
26511 |
| Gene: |
HSPB6 |
More about : HSPB6 |
| Long gene name: |
heat shock protein family B (small) member 6 |
| Synonyms gene name: |
B6 , alpha-crystallin-related, heat shock protein |
| Synonyms name: |
protein phosphatase 1, regulatory subunit 91 |
| Locus: |
19q13, 12 |
| Discovery year: |
2004-05-12 |
| GenBank acession: |
AJ278121 |
| Entrez gene record: |
126393 |
| Pubmed identfication: |
12820654 14717697 |
| RefSeq identity: |
NM_144617 |
| Classification: |
Small heat shock proteins Protein phosphatase 1 regulatory subunits |
| Havana BLAST/BLAT: |
OTTHUMG00000048122 |