| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
DNA Ligase IV/LIG4 |
| Specificity: |
This antibody reacts specifically with DNA Ligase IV/LIG4 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human DNA Ligase IV |
| Gene ID: |
3981 |
| Swiss Prot: |
P49917 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends, Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination, The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4, Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
DNA Ligase IV/LIG4 Polyclonal Antibody |
| Other name: |
Anti-DNA Ligase IV/LIG4 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
DNA Ligase IV/LIG4 |
| Short name: |
Anti-DNA Ligase IV/LIG4 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, Desoxyribonucleic acid, adenosine triphosphate-dependent (polyclonal), antibody to-Desoxyribonucleic acid Ligase IV/ligase IV |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
ATP-dependent, DNA, DNA gap filling and biological process this GO :0006302 and double-strand break repair and biological process this GO :0006303 and double-strand break repair via nonhomolo this GO us end joining and biological process this GO :0006310 and DNA recombination and biological process this GO :0007049 and cell cycle and biological process this GO :0007417 and central nervous system development and biological process this GO :0008022 and protein C-terminus binding and molecular function this GO :0008283 and cell proliferation and biological process this GO :0010165 and response to X-ray and biological process this GO :0010212 and response to ionizing radiation and biological process this GO :0010332 and response to gamma radiation and biological process this GO :0016032 and viral process and biological process this GO :0016874 and ligase activity and molecular function this GO :0032807 and DNA ligase IV complex and cellular component this GO :0033077 and T cell differentiation in thymus and biological process this GO :0033151 and V(D)J recombination and biological process this GO :0033152 and immunoglobulin V(D)J recombination and biological process this GO :0033153 and T cell receptor V(D)J recombination and biological process this GO :0035019 and somatic stem cell maintenance and biological process this GO :0043524 and negative regulation of neuron apoptotic process and biological process this GO :0045190 and isotype switching and biological process this GO :0046872 and metal ion binding and molecular function this GO :0048146 and positive regulation of fibroblast proliferation and biological process this GO :0050769 and positive regulation of neurogenesis and biological process this GO :0051102 and DNA ligation involved in DNA recombination and biological process this GO :0051103 and DNA ligation involved in DNA repair and biological process this GO :0051276 and chromosome organization and biological process this GO :0051301 and cell division and biological process this GO :0051402 and neuron apoptotic process and biological process this GO :0070419 and nonhomolo this GO us end joining complex and cellular component this GO :0071285 and cellular response to lithium ion and biological process this GO :0071897 and DNA biosynthetic process and biological process this GO :0075713 and establishment of integrated proviral latency and biological process, LIG4 and IDBG-49744 and ENSG00000174405 and 3981, LIG4 and IDBG-633610 and ENSBTAG00000015868 and 781252, LIG4S, Lig4 and IDBG-131943 and ENSMUSG00000049717 and 319583, metal ion binding, nuclei, this GO :0000012 and single strand break repair and biological process this GO :0000793 and condensed chromosome and cellular component this GO :0001701 and in utero embryonic development and biological process this GO :0002328 and pro-B cell differentiation and biological process this GO :0003677 and DNA binding and molecular function this GO :0003909 and DNA ligase activity and molecular function this GO :0003910 and DNA ligase (ATP) activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005925 and focal adhesion and cellular component this GO :0005958 and DNA-dependent protein kinase-DNA ligase 4 complex and cellular component this GO :0006266 and DNA ligation and biological process this GO :0006273 and lagging strand elongation and biological process this GO :0006281 and DNA repair and biological process this GO :0006297 and nucleotide-excision repair, this GO :0003677 : DNA binding, this GO :0003677 : DNA binding and also this GO :0003909 : DNA ligase activity and also this GO :0003910 : DNA ligase (ATP) activity and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0008022 : protein C-terminus binding and also this GO :0016874 : ligase activity and also this GO :0046872 : metal ion binding, this GO :0003909 : DNA ligase activity, this GO :0003910 : DNA ligase (ATP) activity, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0008022 : protein C-terminus binding, this GO :0016874 : ligase activity, this GO :0046872 : metal ion binding, ligase IV |
| Identity: |
6601 |
| Gene: |
LIG4 |
More about : LIG4 |
| Long gene name: |
DNA ligase 4 |
| Synonyms gene name: |
ATP-dependent , DNA, ligase IV |
| Synonyms name: |
polydeoxyribonucleotide synthase [ATP] 4 polynucleotide ligase sealase DNA repair enzyme DNA joinase |
| Locus: |
13q33, 3 |
| Discovery year: |
1995-08-10 |
| GenBank acession: |
X83441 |
| Entrez gene record: |
3981 |
| Pubmed identfication: |
7760816 |
| RefSeq identity: |
NM_002312 |
| Classification: |
DNA ligases |
| Havana BLAST/BLAT: |
OTTHUMG00000017328 |
| Locus Specific Databases: |
LIG4base: Mutation registry for LIG4 syndrome LRG_79 |