| Long name: |
Cy3 Conjugated, DNA Ligase IV/LIG4 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
cy3 conjugation kit |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
DNA Ligase IV/LIG4 |
| Specificity: |
This is a highly specific antibody against DNA Ligase IV/LIG4 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
60-110/911 |
| Subcellular location: |
Nucleus |
| Source: |
KLH conjugated synthetic peptide derived from human DNA Ligase IV |
| Gene ID number: |
3981 |
| Swiss Prot: |
P49917 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends, Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination, The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4, Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
512, 550nm/570, 615nm |
| Properties: |
1 of the DNA Ligase IV/LIG4 Antibody, Conjugated can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC, Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser, This Bioss Primary Conjugated Antibodies antibody is well suited for fluorescent microscopy, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
DNA Ligase IV/LIG4 Conjugated |
| Short name: |
Conjugated, DNA Ligase IV/LIG4 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Cy3 |
| Alternative name: |
Desoxyribonucleic acid, adenosine triphosphate-dependent (Antibody to), cyanine 3 coupled, Desoxyribonucleic acid Ligase IV/ligase IV |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
ATP-dependent, DNA, DNA gap filling and biological process this GO :0006302 and double-strand break repair and biological process this GO :0006303 and double-strand break repair via nonhomolo this GO us end joining and biological process this GO :0006310 and DNA recombination and biological process this GO :0007049 and cell cycle and biological process this GO :0007417 and central nervous system development and biological process this GO :0008022 and protein C-terminus binding and molecular function this GO :0008283 and cell proliferation and biological process this GO :0010165 and response to X-ray and biological process this GO :0010212 and response to ionizing radiation and biological process this GO :0010332 and response to gamma radiation and biological process this GO :0016032 and viral process and biological process this GO :0016874 and ligase activity and molecular function this GO :0032807 and DNA ligase IV complex and cellular component this GO :0033077 and T cell differentiation in thymus and biological process this GO :0033151 and V(D)J recombination and biological process this GO :0033152 and immunoglobulin V(D)J recombination and biological process this GO :0033153 and T cell receptor V(D)J recombination and biological process this GO :0035019 and somatic stem cell maintenance and biological process this GO :0043524 and negative regulation of neuron apoptotic process and biological process this GO :0045190 and isotype switching and biological process this GO :0046872 and metal ion binding and molecular function this GO :0048146 and positive regulation of fibroblast proliferation and biological process this GO :0050769 and positive regulation of neurogenesis and biological process this GO :0051102 and DNA ligation involved in DNA recombination and biological process this GO :0051103 and DNA ligation involved in DNA repair and biological process this GO :0051276 and chromosome organization and biological process this GO :0051301 and cell division and biological process this GO :0051402 and neuron apoptotic process and biological process this GO :0070419 and nonhomolo this GO us end joining complex and cellular component this GO :0071285 and cellular response to lithium ion and biological process this GO :0071897 and DNA biosynthetic process and biological process this GO :0075713 and establishment of integrated proviral latency and biological process, LIG4 and IDBG-49744 and ENSG00000174405 and 3981, LIG4 and IDBG-633610 and ENSBTAG00000015868 and 781252, LIG4S, Lig4 and IDBG-131943 and ENSMUSG00000049717 and 319583, metal ion binding, nuclei, this GO :0000012 and single strand break repair and biological process this GO :0000793 and condensed chromosome and cellular component this GO :0001701 and in utero embryonic development and biological process this GO :0002328 and pro-B cell differentiation and biological process this GO :0003677 and DNA binding and molecular function this GO :0003909 and DNA ligase activity and molecular function this GO :0003910 and DNA ligase (ATP) activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005925 and focal adhesion and cellular component this GO :0005958 and DNA-dependent protein kinase-DNA ligase 4 complex and cellular component this GO :0006266 and DNA ligation and biological process this GO :0006273 and lagging strand elongation and biological process this GO :0006281 and DNA repair and biological process this GO :0006297 and nucleotide-excision repair, this GO :0003677 : DNA binding, this GO :0003677 : DNA binding and also this GO :0003909 : DNA ligase activity and also this GO :0003910 : DNA ligase (ATP) activity and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0008022 : protein C-terminus binding and also this GO :0016874 : ligase activity and also this GO :0046872 : metal ion binding, this GO :0003909 : DNA ligase activity, this GO :0003910 : DNA ligase (ATP) activity, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0008022 : protein C-terminus binding, this GO :0016874 : ligase activity, this GO :0046872 : metal ion binding, ligase IV |
| Identity: |
6601 |
| Gene: |
LIG4 |
More about : LIG4 |
| Long gene name: |
DNA ligase 4 |
| Synonyms gene name: |
ATP-dependent , DNA, ligase IV |
| Synonyms name: |
polydeoxyribonucleotide synthase [ATP] 4 polynucleotide ligase sealase DNA repair enzyme DNA joinase |
| Locus: |
13q33, 3 |
| Discovery year: |
1995-08-10 |
| GenBank acession: |
X83441 |
| Entrez gene record: |
3981 |
| Pubmed identfication: |
7760816 |
| RefSeq identity: |
NM_002312 |
| Classification: |
DNA ligases |
| Havana BLAST/BLAT: |
OTTHUMG00000017328 |
| Locus Specific Databases: |
LIG4base: Mutation registry for LIG4 syndrome LRG_79 |