| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
HIC1 |
| Specificity: |
This antibody reacts specifically with HIC1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human HIC1 |
| Gene ID: |
3090 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Conversely, HIC-1 does not interact with components of the HDAC complex, HIC-1 is deleted in the genetic disorder Miller-Dieker syndrome (MDS), HIC-1 is ubiquitously expressed in adult tissues and its structure is defined by five zinc fingers and an N-terminal broad complex POZ (or BTB) domain, In several BTB/POZ containing proteins, These and other studies indicate that HIC-1 acts as a putative tumor suppressor protein that mediates transcriptional repression, When this domain is deleted, and the expression of HIC-1 is also frequently suppressed in leukemia and various cancers due to the hypermethylation of specific DNA regions and the resulting transcriptional silencing, as with the oncogenic PLZF-RAR chimera of promyelocytic leukemias, including BCL-6 and the promyelocytic leukemia zinc-finger (PLZF) oncoprotein, suggesting that HIC-1-induced transcriptional repression is unassociated with the POZ/BTB domain, this domain interacts with the SMRT/N-CoR-mSin3A HDAC complex and is directly involved in repressing and silencing gene transcription, this transcriptional repression is attenuated, Hypermethylated in cancer (HIC-1) was originally identified as a target of p53-induced gene expression |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
ZBTB29 ZNF901 |
| Also known as: |
HIC1 Polyclonal Antibody |
| Other name: |
Anti-HIC1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
HIC1 |
| Short name: |
Anti-HIC1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-HIC1 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
4909 |
| Gene: |
HIC1 |
More about : HIC1 |
| Long gene name: |
HIC ZBTB transcriptional repressor 1 |
| Synonyms gene name: |
hypermethylated in cancer 1 |
| Locus: |
17p13, 3 |
| Discovery year: |
1996-03-19 |
| Entrez gene record: |
3090 |
| RefSeq identity: |
NM_006497 |
| Classification: |
BTB domain containing Zinc fingers C2H2-type |
| Havana BLAST/BLAT: |
OTTHUMG00000177635 |