| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Nck1/NCK alpha |
| Specificity: |
This antibody reacts specifically with Nck1/NCK alpha |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Nck2 |
| Gene ID: |
4690 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
It is a common target for the action of different surface receptors, Like other SH2 containing proteins, Nck is associated with tyrosine autophosphorylated EGF or PDGF receptors via its SH2 domain, Nck is phosphorylated on tyrosine, Nck is widely expressed in various tissues and in cell lines from human, Overexpression of Nck leads to transformation of NIH 3T3 cells, Ras GTPase activating protein, and phospholipase Cg, and rat origins, and threonine residues in response to stimulation of EGF and PDGF in A431 and NIH 3T3 cells respectively, encoding one SH2 and three SH3 domains, murine, oncogenic protein, phosphatidylinositol 3 kinase, serine, the Src homology motifs found in nonreceptor tyrosine kinases, Nck is a highly conserved |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
NCKalpha |
| Also known as: |
Nck1/NCK alpha Polyclonal Antibody |
| Other name: |
Anti-Nck1/NCK alpha Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Nck1/NCK alpha |
| Short name: |
Anti-Nck1/NCK alpha (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-NCK adaptor protein 1/NCK a (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
7664 |
| Gene: |
NCK1 |
More about : NCK1 |
| Long gene name: |
NCK adaptor protein 1 |
| Synonyms gene: |
NCK |
| Locus: |
3q22, 3 |
| Discovery year: |
1998-11-04 |
| GenBank acession: |
X17576 |
| Entrez gene record: |
4690 |
| Pubmed identfication: |
7806213 9737977 |
| RefSeq identity: |
NM_006153 |
| Classification: |
SH2 domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000159781 |