| Long name: |
FITC Conjugated, Nck1/NCK alpha Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Nck1/NCK alpha |
| Specificity: |
This is a highly specific antibody against Nck1/NCK alpha |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human Nck2 |
| Gene ID number: |
4690 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
It is a common target for the action of different surface receptors, Like other SH2 containing proteins, Nck is associated with tyrosine autophosphorylated EGF or PDGF receptors via its SH2 domain, Nck is phosphorylated on tyrosine, Nck is widely expressed in various tissues and in cell lines from human, Overexpression of Nck leads to transformation of NIH 3T3 cells, Ras GTPase activating protein, and phospholipase Cg, and rat origins, and threonine residues in response to stimulation of EGF and PDGF in A431 and NIH 3T3 cells respectively, encoding one SH2 and three SH3 domains, murine, oncogenic protein, phosphatidylinositol 3 kinase, serine, the Src homology motifs found in nonreceptor tyrosine kinases, Nck is a highly conserved |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
NCKalpha |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Nck1/NCK alpha Conjugated |
| Short name: |
FITC Conjugated, Nck1/NCK alpha Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, NCK adaptor protein 1/NCK a (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
7664 |
| Gene: |
NCK1 |
More about : NCK1 |
| Long gene name: |
NCK adaptor protein 1 |
| Synonyms gene: |
NCK |
| Locus: |
3q22, 3 |
| Discovery year: |
1998-11-04 |
| GenBank acession: |
X17576 |
| Entrez gene record: |
4690 |
| Pubmed identfication: |
7806213 9737977 |
| RefSeq identity: |
NM_006153 |
| Classification: |
SH2 domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000159781 |