| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
GAL4 |
| Specificity: |
This antibody reacts specifically with GAL4 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from Saccharomyces cerevisiae GAL4 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Yeast |
| Cross Reactive Species details: |
Saccharomyces cerevisiae |
| Background information: |
As the N-terminal 147 amino acid residues of GAL4 are sufficient to mediate specific and strong binding to DNA, Fields and coworkers have taken advantage of these findings by the development of a unique â, Fusions between GAL4 (amino acids 1-147) and activating domains from a variety of transcriptional regulatory proteins can activate transcription in yeast, This approach is facilitated by the finding that higher eukaryotes lack endogenous proteins that enhance transcription from the consensus GAL4-binding site, but are incapable of efficient transcriptional activation, insects and mammalian cells, plant, this protein fragment has frequently been used to confer specific DNA binding in experiments examining transcriptional activation functions of heterologous proteins, &euro, &euro, &oelig, The GAL4 protein of Saccharomyces cerevisiae is one of the most thoroughly characterized transcriptional activators, two-hybridâ, system using GAL4 fusions in yeast to identify specific protein-protein interactions |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
GAL4 |
| Also known as: |
GAL4 Polyclonal Antibody |
| Other name: |
Anti-GAL4 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
GAL4 |
| Short name: |
Anti-GAL4 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-GAL4 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
6565 |
| Gene: |
LGALS4 |
More about : LGALS4 |
| Long gene name: |
galectin 4 |
| Synonyms gene name: |
4 , galactoside-binding, lectin, soluble |
| Locus: |
19q13, 2 |
| Discovery year: |
1993-08-24 |
| Entrez gene record: |
3960 |
| Pubmed identfication: |
8063692 |
| RefSeq identity: |
NM_006149 |
| Classification: |
Galectins |
| Havana BLAST/BLAT: |
OTTHUMG00000182608 |