| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
NOSTRIN |
| Specificity: |
This antibody reacts specifically with NOSTRIN |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human NOSTRIN |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
It consists of an N-terminal Cdc15 domain with an FCH (Fes/CIP homology) region, It functions as an adaptor protein binding to caveolin-1 via an internal domain and NOS3 via its SH3 domain, NOSTRIN also interacts with Dynamin and N-WASP via its SH3 domain, NOSTRIN is expressed in the vascular endothelial cells of highly vascularized tissues such as placenta, NOSTRIN typically exists as a trimer, The NOS3 protein is responsible for the production of nitric oxide (NO), The translocation of NOS3 from the plasma membrane to intracellular vesicle-like structures diminishes NO production, a potent mediator in various biological processes, also known as endothelial nitric oxide synthase traffic inducer, forming a ternary complex which facilitates caveolar transport of NOS3, is a member of the Pombe Cdc15 homology (PCH) family of proteins, kidney and heart, lung, two coiled coil domains and a C-terminal SH3 domain, NOSTRIN (nitric oxide synthase trafficker isoform 1) |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
MGC20702 |
| Also known as: |
NOSTRIN Polyclonal Antibody |
| Other name: |
Anti-NOSTRIN Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
NOSTRIN |
| Short name: |
Anti-NOSTRIN (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-nitric oxide synthase trafficker (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
BT, DNA-templated and biological process this GO :0046209 and nitric oxide metabolic process and biological process this GO :0050999 and regulation of nitric-oxide synthase activity and biological process, NOSTRIN and IDBG-74325 and ENSG00000163072 and 115677, Nostrin and IDBG-175946 and ENSMUSG00000034738 and 329416, nuclei, protein binding, this GO :0003677 and DNA binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005856 and cytoskeleton and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006897 and endocytosis and biological process this GO :0007165 and signal transduction and biological process this GO :0030666 and endocytic vesicle membrane and cellular component this GO :0044281 and small molecule metabolic process and biological process this GO :0045892 and negative regulation of transcription, this GO :0003677 : DNA binding, this GO :0003677 : DNA binding and also this GO :0005515 : protein binding, this GO :0005515 : protein binding, 17865 and IDBG-641032 and ENSBTAG00000010362 and 521834, nitric oxide synthase trafficker |
| Identity: |
20203 |
| Gene: |
NOSTRIN |
More about : NOSTRIN |
| Long gene name: |
nitric oxide synthase trafficking |
| Synonyms gene name: |
nitric oxide synthase trafficker |
| Locus: |
2q24, 3 |
| Discovery year: |
2003-01-06 |
| GenBank acession: |
AJ532842 |
| Entrez gene record: |
115677 |
| Pubmed identfication: |
12446846 |
| RefSeq identity: |
NM_052946 |
| Classification: |
F-BAR domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000153990 |