| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
GRAP |
| Specificity: |
This antibody reacts specifically with GRAP |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human GRAP |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
For instance, GRB10 (also referred to as GRB-IR), GRB14 and Grap (for GRB2-related adapter protein), GRB7, Grap also associates with the Ras guanine nucleotide exchange factor Sos 1 via its amino terminal SH3 domain, Grap shares the highest degree of homology with GRB2 exhibiting 59% sequence identity with GRB2, Members include GRB2, Signaling by such receptors involves a series of intermediates characterized by SH2 domains that bind tyrosine phosphorylated receptors by a direct interaction between the SH2 domain and specific receptor sequences, The Grap SH2 domain is capable of binding to the activated stem cell factor receptor, While GRB10 and GRB14 are most closely related to GRB7, c-Kit and the erythropoietin receptor (EpoR), the GRB family of proteins lack a defined catalytic activity and are entirely composed of SH2 and SH3 domains, Many growth factors function by binding receptors with intrinsic tyrosine kinase activity |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
GRAP Polyclonal Antibody |
| Other name: |
Anti-GRAP Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
GRAP |
| Short name: |
Anti-GRAP (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-GRAP (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
4562 |
| Gene: |
GRAP |
More about : GRAP |
| Long gene name: |
GRB2-related adaptor protein |
| Locus: |
17p11, 2 |
| Discovery year: |
1998-09-29 |
| GenBank acession: |
U52518 |
| Entrez gene record: |
10750 |
| Pubmed identfication: |
8647802 8995379 |
| RefSeq identity: |
NM_006613 |
| Classification: |
SH2 domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000059436 |