| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
I30 |
| Specificity: |
This antibody reacts specifically with IFI30 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human IFI30 |
| Gene ID: |
10437 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
A lysosomal thiol reductase, Additionally, Cytosolic reduction is mediated enzymatically by thioredoxin, Efficient proteolysis requires denaturation, GILT, GILT is expressed constitutively in antigen-presenting cells and is induced by g-interferon in other cell types, GILT is synthesized as a precursor, In the endocytic pathway, T cell recognition of select exogenous and endogenous epitopes is dependent on tumor cell expression of GILT, The absence of GILT in melanomas alters antigen processing and the hierarchy of immunodominant epitope presentation, This process is catalyzed by a gamma-interferon-inducible thiol reductase (GILT), and following delivery to MHC class II-containing compartments (MIICs), and reduction of inter- and intrachain disulfide bonds, induced by acidic conditions within lysosomes, is optimally active at low pH and capable of catalyzing disulfide bond reduction both in vivo and in vitro, is processed to the mature form via cleavage of amino- and carboxy-terminal propeptides, reduction of protein disulfide bonds is important for the generation of MHC class II-peptide complexes, suggesting a potentially important role in antigen processing, Proteins internalized into the endocytic pathway are usually degraded |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
IFI-30 GILT IP30 MGC32056 IP-30 |
| Also known as: |
IFI30 Polyclonal Antibody |
| Other name: |
Anti-IFI30 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
IFI30 |
| Short name: |
Anti-IFI30 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, gamma-inducible protein 30 (polyclonal), antibody to-interferon |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
Extracellular, GILT and IFI-30 and IP30, IFI30 and IDBG-281623 and ENSG00000216490 and 10437, IFI30 and IDBG-641754 and ENSBTAG00000018349 and 615930, Ifi30 and IDBG-164061 and ENSMUSG00000031838 and 65972, acting on a sulfur group of donors, acting on a sulfur group of donors, acting on a sulfur group of donors and molecular function this GO :0019221 and cytokine-mediated signaling pathway and biological process this GO :0019886 and antigen processing and presentation of exogenous peptide antigen via MHC class II and biological process this GO :0030054 and cell junction and cellular component this GO :0042590 and antigen processing and presentation of exogenous peptide antigen via MHC class I and biological process this GO :0043202 and lysosomal lumen and cellular component this GO :0043231 and intracellular membrane-bounded organelle and cellular component this GO :0048147 and negative regulation of fibroblast proliferation and biological process this GO :0050821 and protein stabilization and biological process this GO :0055114 and oxidation-reduction process and biological process this GO :0060333 and interferon-gamma-mediated signaling pathway and biological process, gamma-inducible protein 30, oxidoreductase activity, this GO :0005576 and extracellular region and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005764 and lysosome and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0016667 and oxidoreductase activity, this GO :0016667 : oxidoreductase activity, this GO :0016667 : oxidoreductase activity, interferon |
| Identity: |
5398 |
| Gene: |
IFI30 |
More about : IFI30 |
| Long gene name: |
IFI30, lysosomal thiol reductase |
| Synonyms gene name: |
interferon gamma inducible protein 30 |
| Synonyms name: |
gamma-interferon-inducible lysosomal thiol reductase interferon gamma-inducible protein 30 preproprotein legumaturain |
| Locus: |
19p13, 11 |
| Discovery year: |
1999-12-09 |
| GenBank acession: |
J03909 |
| Entrez gene record: |
10437 |
| Pubmed identfication: |
3136170 10639150 |
| RefSeq identity: |
NM_006332 |
| Havana BLAST/BLAT: |
OTTHUMG00000183373 |