| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
GATA4 Ser105 |
| Specificity: |
This antibody reacts specifically with GATA4 (Ser105) |
| Modification: |
Phosphorylation |
| Modification site: |
Ser105 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human GATA4 around the phosphorylation site of Ser105 |
| Gene ID: |
2626 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
AGATCTTA, Although expression patterns of the various GATA transcription factors may overlap, Both GATA-4 and GATA-6 are found in heart, GATA-1 is erythroid-specific and is responsible for the regulated transcription of erythroid genes, GATA-2 is expressed in embryonic brain and liver, GATA-3 is highly expressed in T lymphocytes, GATA-4, GATA-5 and GATA-6 comprise a subfamily of transcription factors, GATA-5 expression has been observed in differentiated heart and gut tissues and is present throughout the course of development in the heart, HeLa and endothelial cells, It is an essential component in the generation of the erythroid lineage, Studies with a modified GATA consensus sequence, This indicates broader regulatory capabilities of GATA-2 and GATA-3 than GATA-1, as well as in erythroid cells, but not GATA-4 expression, have shown that GATA-2 and GATA-3 recognize this mutated consensus while GATA-1 has poor recognition of this sequence, it is not yet apparent how the GATA factors are able to discriminate in binding their appropriate target sites, lung and liver tissues exhibit GATA-6, pancreas and ovary, Members of the GATA family share a conserved zinc finger DNA-binding domain and are capable of binding the WGATAR consensus sequence |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
GATA4 (Ser105) Polyclonal Antibody |
| Other name: |
Anti-GATA4 (Ser105) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
GATA4 (Ser105) |
| Short name: |
Anti-GATA4 (Ser105) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-GATA4 (Ser105) (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
4173 |
| Gene: |
GATA4 |
More about : GATA4 |
| Long gene name: |
GATA binding protein 4 |
| Synonyms gene name: |
GATA-binding protein 4 |
| Locus: |
8p23, 1 |
| Discovery year: |
1994-11-30 |
| GenBank acession: |
AK097060 |
| Entrez gene record: |
2626 |
| Pubmed identfication: |
7665171 |
| RefSeq identity: |
NM_002052 |
| Classification: |
GATA zinc finger domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000090800 |