| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
RASA1 Tyr460 |
| Specificity: |
This antibody reacts specifically with RASA1 (Tyr460) |
| Modification: |
Phosphorylation |
| Modification site: |
Tyr460 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic phosphopeptide derived from human GAP around the phosphorylation site of Tyr460 |
| Gene ID: |
5921 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Interaction of p21Ras with GTPase activating protein (GAP) can increase hydrolysis of p21Ras-bound GTP by as much as 1000-fold (4, Oncogenic p21Ras proteins are trapped in the excited signal-emitting state because the mechanism normally employed to delimit their excitation period, Rab 3A and Rap 1, The product of the neurofibromatosis type 1 gene (NF1) has also been shown to exhibit p21Ras GAP activity (6, and proteins that stimulate the GTPase activity of three other low molecular weight GTPases, c-K- and N-Ras proto-oncogenes encode ubiquitously expressed proteins (1, have also been described (8, hydrolysis of their bound GTP to GDP, including Rho, is impaired as a result of specific mutations (3), p21Ras can exist in either a physiologically quiescent GDP-binding state or a GTP-binding signal-emitting state (3), 2), 5), 7), 9), The mammalian c-H- |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
GAP CM-AVM p120GAP p120RASGAP p120 |
| Also known as: |
RASA1 (Tyr460) Polyclonal Antibody |
| Other name: |
Anti-RASA1 (Tyr460) Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
RASA1 (Tyr460) |
| Short name: |
Anti-RASA1 (Tyr460) (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-RAS p21 protein activator (GTPase activating protein) 1 (Tyr460) (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
BT, CM-AVM and CMAVM and GAP and p120GAP and p120RASGAP and PKWS and RASA and RASGAP, GTPase binding, Plasma membranes, RASA1 and IDBG-32785 and ENSG00000145715 and 5921, Rasa1 and IDBG-170464 and ENSMUSG00000021549 and 218397, this GO :0000281 and mitotic cytokinesis and biological process this GO :0001570 and vasculogenesis and biological process this GO :0001726 and ruffle and cellular component this GO :0001948 and glycoprotein binding and molecular function this GO :0001953 and negative regulation of cell-matrix adhesion and biological process this GO :0005096 and GTPase activator activity and molecular function this GO :0005099 and Ras GTPase activator activity and molecular function this GO :0005102 and receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005622 and intracellular and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0007162 and negative regulation of cell adhesion and biological process this GO :0007165 and signal transduction and biological process this GO :0008360 and regulation of cell shape and biological process this GO :0009790 and embryo development and biological process this GO :0019870 and potassium channel inhibitor activity and molecular function this GO :0030833 and regulation of actin filament polymerization and biological process this GO :0031235 and intrinsic component of the cytoplasmic side of the plasma membrane and cellular component this GO :0032320 and positive regulation of Ras GTPase activity and biological process this GO :0035556 and intracellular signal transduction and biological process this GO :0043524 and negative regulation of neuron apoptotic process and biological process this GO :0043547 and positive regulation of GTPase activity and biological process this GO :0046580 and negative regulation of Ras protein signal transduction and biological process this GO :0051020 and GTPase binding and molecular function this GO :0051056 and regulation of small GTPase mediated signal transduction and biological process this GO :0051252 and regulation of RNA metabolic process and biological process, this GO :0001948 : glycoprotein binding, this GO :0001948 : glycoprotein binding and also this GO :0005096 : GTPase activator activity and also this GO :0005099 : Ras GTPase activator activity and also this GO :0005102 : receptor binding and also this GO :0005515 : protein binding and also this GO :0019870 : potassium channel inhibitor activity and also this GO :0051020 : GTPase binding, this GO :0005096 : GTPase activator activity, this GO :0005099 : Ras GTPase activator activity, this GO :0005102 : receptor binding, this GO :0005515 : protein binding, this GO :0019870 : potassium channel inhibitor activity, this GO :0051020 : GTPase binding, 102247 and IDBG-646529 and ENSBTAG00000009565 and 282032, RAS p21 protein activator (GTPase activating protein) 1 |
| Identity: |
9871 |
| Gene: |
RASA1 |
More about : RASA1 |
| Long gene name: |
RAS p21 protein activator 1 |
| Synonyms gene: |
RASA |
| Synonyms gene name: |
RAS p21 protein activator (GTPase activating protein) 1 |
| Synonyms name: |
capillary malformation-arteriovenous malformation p120 RAS GTPase activating protein |
| Locus: |
5q14, 3 |
| Discovery year: |
1989-06-30 |
| Entrez gene record: |
5921 |
| Pubmed identfication: |
15917201 |
| RefSeq identity: |
NM_002890 |
| Classification: |
Pleckstrin homology domain containing SH2 domain containing C2 and RasGAP domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000162605 |
| Locus Specific Databases: |
SH2base: Database for pathogenic SH2 domain mutations |