| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
GALNT1/GalNAc-T1 |
| Specificity: |
This antibody reacts specifically with GALNT1/GalNAc-T1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human GALNT1/GalNAc-T1 |
| Gene ID: |
2589 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Due to alternative splicing events, GalNAc-T1, GalNAc-T1 catalyzes the first reaction in O-linked oligosaccharide biosynthesis, GalNAc-T1 uses calcium and manganese as cofactors, also known as GALNT1 (Polypeptide N-acetylgalactosaminyltransferase 1), contains a stem region and a C-terminal ricin/lectin-like domain, is a ubiquitously expressed 559 amino acid single-pass type II membrane protein that localizes to the Golgi apparatus and, like other GalNAc-Ts, namely the transfer of an N-acetyl-D-galactosamine residue to a protein acceptor, thereby initiating mucin-type O-linked glycosylation in the Golgi apparatus, two GalNAc-T1 isoforms are expressed, The UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes are substrate-specific proteins that catalyze the transfer of GalNAc (N-acetylgalactosamine) to serine and threonine residues onto various proteins |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
GalNAc-T1 |
| Also known as: |
GALNT1/GalNAc-T1 Polyclonal Antibody |
| Other name: |
Anti-GALNT1/GalNAc-T1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
GALNT1/GalNAc-T1 |
| Short name: |
Anti-GALNT1/GalNAc-T1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-GALNT1/GalNAc-T1 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
4123 |
| Gene: |
GALNT1 |
More about : GALNT1 |
| Long gene name: |
polypeptide N-acetylgalactosaminyltransferase 1 |
| Synonyms gene name: |
UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1) |
| Synonyms name: |
protein-UDP acetylgalactosaminyltransferase 1 polypeptide GalNAc transferase 1 |
| Locus: |
18q12, 2 |
| Discovery year: |
1996-10-26 |
| Entrez gene record: |
2589 |
| Pubmed identfication: |
7592619 12199709 |
| RefSeq identity: |
NM_020474 |
| Classification: |
Polypeptide N-acetylgalactosaminyltransferases |
| Havana BLAST/BLAT: |
OTTHUMG00000132567 |