| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Dynamin 1 |
| Specificity: |
This antibody reacts specifically with Dynamin 1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Dynamin 1 |
| Gene ID: |
1759 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Dephosphorylation is required for synaptic vesicle retrieval, Dynamin I is phosphorylated in nerve terminals exclusively in the cytosolic compartment and in vitro by protein kinase C (PKC) (2â, Mouse, The phosphorylation site in PKC-phosphorylated Dynamin I is a single site at Serine 795, by Cdk5, including Ser 778, rat and human Dynamin I are phosphorylated on serine residues, regulating PACSIN1 recruitment and enabling synaptic vesicle endocytosis, suggesting that phosphorylation affects the subcellular localization of Dynamin I (5), the regulatory subunit of phosphatidylinositol 3-kinase (2â, which is located near a binding site for the SH3 domain of p85, &euro, &euro, &ldquo, &ldquo, 5), 5), Dynamin I is a GTPase enzyme required for the retrieval of synaptic vesicles after exocytosis and functions in endocytosis by stimulating assembly of invaginating synaptic vesicles (1) |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
Dynamin 1 Polyclonal Antibody |
| Other name: |
Anti-Dynamin 1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Dynamin 1 |
| Short name: |
Anti-Dynamin 1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Dynamin 1 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
2972 |
| Gene: |
DNM1 |
More about : DNM1 |
| Long gene name: |
dynamin 1 |
| Synonyms gene: |
DNM |
| Locus: |
9q34, 11 |
| Discovery year: |
1992-07-09 |
| GenBank acession: |
L07807 |
| Entrez gene record: |
1759 |
| Pubmed identfication: |
2144893 9143509 |
| RefSeq identity: |
NM_004408 |
| Classification: |
Pleckstrin homology domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000020733 |