| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
GAD67/GAD1 |
| Specificity: |
Belongs to the group II decarboxylase family, GAD67 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human GAD67 |
| Gene ID: |
2571 |
| Swiss Prot: |
Q99259 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
(585 amino acid residues) and is encoded on human chromosome 10, GAD exists as two isoforms, GAD has a restricted tissue distribution, GAD is also transiently expressed in non-GABAergic cells of the embryonic and adult nervous system, GAD65 and GAD67 (molecular masses of 65 and 67 kD, GAD65 has been identified as an autoantigen in insulindependent diabetes mellitus (IDDM) and stiff-man syndrome (SMS), GAD65 is an ampiphilic, GAD67 is a cytoplasmic protein (594 amino acid residues) and is encoded on chromosome 2, IDDM is an autoimmune disease that results from T cell mediated destruction of pancreatic insulin-secreting beta cells, Islet-reactive T cells and primarily to GAD65 (also named beta cell autoantigen) can be detected in peripheral blood of 80% of recent-onset IDD patients and in pre-diabetic high-risk subjects before onset of clinical symptoms, It is also present in other non-neuronal tissues such as testis, It is highly expressed in the cytoplasm of GABAergic neurons in the central nervous system (CNS) and pancreatic beta cells, The two isoforms appear to have distinct intraneuronal distribution in the brain, There is 64% amino acid identity between the two isoforms, This suggests that GAD may be an important marker in the early stages of the disease, and a putative paracrine signal molecule in pancreatic islets, membraneanchored protein, oviduct and ovary, respectively) that are encoded by two different genes, suggesting its involvement in development and plasticity, the principal inhibitory neurotransmitter in the brain, which in GAD65 is required for targeting the enzyme to GABA-containing secretory vesicles, with the highest diversity located at the N terminus, Glutamic Acid Decarboxylase (GAD) catalyzes the conversion of L glutamate to g-aminobutyric acid (GABA) |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
67 kDa glutamic acid decarboxylase, CPSQ1, GAD-67, GAD1, GAD67, Glutamate decarboxylase 1, Glutamate decarboxylase 67 kDa isoform, SCP, GAD |
| Also known as: |
GAD67 Polyclonal Antibody |
| Other name: |
Anti-GAD67 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
GAD67 |
| Short name: |
Anti-GAD67 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-GAD67 (polyclonal) |
| Alternative technique: |
polyclonals |