| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Dnmt2 |
| Specificity: |
This antibody reacts specifically with Dnmt2 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Dnmt2 |
| Gene ID: |
1787 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Additional mammalian Dnmt proteins include Dnmt2 and Dnmt3, DNA methylation requires the enzymatic activity of DNA 5-cytosine methyltransferase (Dnmt) proteins, Dnmt1 is the most abundant, Dnmt2 lacks the large N-terminal regulator domain of Dnmt1, Dnmt3a and Dnmt3b are encoded by two distinct genes, The Dnmt enzymes are encoded by independent genes, and is likely involved in methylating newly integrated retroviral DNA, and it preferentially methylates hemimethylated DNA and coordinates gene expression during development, and this methylation results in transcriptional repression of the target gene, but both are abundantly expressed in embryonic stem cells, is expressed at substantially lower levels in adult tissues, where they also methylate CpG motifs on DNA, which catalyze the transfer of a methyl group from S-adenosyl methionine to the 5'-position of cytosines residing in the dinucleotide CpG motif, Methylation at the 5'-position of cytosine is the only known naturally occurring covalent modification of the mammalian genome |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
RNMT1 |
| Also known as: |
Dnmt2 Polyclonal Antibody |
| Other name: |
Anti-Dnmt2 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Dnmt2 |
| Short name: |
Anti-Dnmt2 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Dnmt2 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
2977 |
| Gene: |
TRDMT1 |
More about : TRDMT1 |
| Long gene name: |
tRNA aspartic acid methyltransferase 1 |
| Synonyms gene: |
DNMT2 |
| Synonyms gene name: |
DNA (cytosine-5-)-methyltransferase 2 |
| Synonyms name: |
tRNA (cytosine(38)-C(5))-methyltransferase |
| Locus: |
10p13 |
| Discovery year: |
1998-05-07 |
| GenBank acession: |
AJ223333 |
| Entrez gene record: |
1787 |
| Pubmed identfication: |
9425235 9763678 16424344 |
| RefSeq identity: |
NM_004412 |
| Havana BLAST/BLAT: |
OTTHUMG00000017745 |