| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
TIE2/CD202b |
| Specificity: |
This antibody reacts specifically with TIE2/CD202b |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Extracellular |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Tie2 |
| Gene ID: |
7010 |
| Swiss Prot: |
Q02763 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
ANGPT1 oligomers recruit TEK to cell-cell contacts, ANGPT1 signaling triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors, ANGPT2 and ANGPT4 and regulates angiogenesis, ANGT1 recruits TEK to contacts with the extracellular matrix, After birth, GRB14, GRB7, Has anti-inflammatory effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels, In migrating endothelial cells that lack cell-cell adhesions, In quiescent vessels, Inhibits angiogenesis and promotes vascular stability in quiescent vessels, PIK3R1, Required for normal angiogenesis and heart development during embryogenesis, Required for post-natal hematopoiesis, SHC1 and TIE1, Signaling is also modulated by formation of heterodimers with TIE1, Signaling is modulated by ANGPT2 that has lower affinity for TEK, TEK phosphorylates DOK2, The soluble extracellular domain modulates signaling by functioning as decoy receptor for angiopoietins, activates or inhibits angiogenesis, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, adhesion and cell spreading, and by proteolytic processing that gives rise to a soluble TEK extracellular domain, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades, and ultimately to the stimulation of sprouting angiogenesis, but also maintenance of vascular quiescence, but inhibits ANGPT1-mediated signaling by competing for the same binding site, can promote TEK autophosphorylation in the absence of ANGPT1, depending on the context, endothelial cell survival, forming complexes with TEK molecules from adjoining cells, leading to the formation of focal adhesion complexes, migration, proliferation, reorganization of the actin cytoskeleton, where endothelial cells have tight contacts, Tyrosine-protein kinase that acts as cell-surface receptor for ANGPT1 |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
TIE2 TIE-2 VMCM1 CD202b |
| Also known as: |
TIE2/CD202b Polyclonal Antibody |
| Other name: |
Anti-TIE2/CD202b Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
TIE2/CD202b |
| Short name: |
Anti-TIE2/CD202b (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-TIE2/CD202b (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
11724 |
| Gene: |
TEK |
More about : TEK |
| Long gene name: |
TEK receptor tyrosine kinase |
| Synonyms gene: |
VMCM |
| Synonyms gene name: |
endothelial , multiple cutaneous and mucosal TEK tyrosine kinase, venous malformations |
| Locus: |
9p21, 2 |
| Discovery year: |
1994-05-24 |
| GenBank acession: |
L06139 |
| Entrez gene record: |
7010 |
| Pubmed identfication: |
1312667 7833915 |
| Classification: |
Fibronectin type III domain containing CD molecules Immunoglobulin like domain containing Receptor Tyrosine Kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000019712 |