| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
NLE1 |
| Specificity: |
This antibody reacts specifically with NLE1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human NLE1 |
| Gene ID: |
54475 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Deletion of the NLE1 gene in mice during the early stages of development results in embryonic death, In plants, It may also play a role in 60S ribosomal subunit biogenesis in yeast, NLE1 contains eight WD40 domains and produces one isoform due to alternative splicing, NLE1 is a 485 amino acid WD40-repeat protein that binds to the cytoplasmic domain of Notch, NLE1 is crucial for normal cellular growth and development, Notch receptors play an important role in development and cell-fate decisions, Notchless is a loss-of-function mutant allele that encodes for protein NLE1 (notchless homolog 1), Under-expression during shoot proliferation causes pleiotropic defects such as delayed flowering and abnormal organ maturation, regulating its signaling activity in Drosophila melanogaster and in mice, while gene deletion in the late stages of development leads to activation of a caspase-3-dependent apoptotic pathway, The Notch signaling pathway is an evolutionary conserved system that is involved in intracellular communication |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
FLJ10458 |
| Also known as: |
NLE1 Polyclonal Antibody |
| Other name: |
Anti-NLE1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
NLE1 |
| Short name: |
Anti-NLE1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-NLE1 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
19889 |
| Gene: |
NLE1 |
More about : NLE1 |
| Long gene name: |
notchless homolog 1 |
| Synonyms gene name: |
notchless homolog 1 (Drosophila) |
| Synonyms name: |
(Drosophila) , Notchless gene homolog |
| Locus: |
17q12 |
| Discovery year: |
2005-05-24 |
| Entrez gene record: |
54475 |
| RefSeq identity: |
NM_018096 |
| Classification: |
WD repeat domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000132928 |