| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
SIX2 |
| Specificity: |
This antibody reacts specifically with SIX2 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human SIX2 |
| Gene ID: |
10736 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
It has been demonstrated that alterations to homeobox-containing genes may result in cancer, Six1 and Six4 are both capable of transactivating MEF3 site containing reporter genes, Six1 expression has been shown to be absent or low in normal adult tissues, Six1 overexpression has been shown to abrogate the G2 cell cycle checkpoint, Six2, Six2 is highly expressed in fetal tissues but expression is limited in adult tissues, Six4 (AREC3) and Six5 bind to the same DNA sequence, although it is expressed in several tumor types, including breast carcinoma, indicating that they may regulate the same target genes, such as myogenin, The Six proteins (sine oculis) are a family of homeodomain transcription factors that share a conserved DNA binding domain |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
SIX2 Polyclonal Antibody |
| Other name: |
Anti-SIX2 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
SIX2 |
| Short name: |
Anti-SIX2 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-SIX2 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
10888 |
| Gene: |
SIX2 |
More about : SIX2 |
| Long gene name: |
SIX homeobox 2 |
| Synonyms gene name: |
sine oculis homeobox (Drosophila) homolog 2 sine oculis homeobox homolog 2 (Drosophila) |
| Locus: |
2p21 |
| Discovery year: |
1999-04-15 |
| GenBank acession: |
AF136939 |
| Entrez gene record: |
10736 |
| Classification: |
SINE class homeoboxes |
| Havana BLAST/BLAT: |
OTTHUMG00000152421 |