| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Ack1 |
| Specificity: |
This antibody reacts specifically with Ack1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cell membrane, Nucleus, Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Ack1 |
| Gene ID: |
10188 |
| Swiss Prot: |
Q07912 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
AR, Activates AKT1 by phosphorylating it on 'Tyr-176', Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, Confers metastatic properties on cancer cells and promotes tumor growth by negatively regulating tumor suppressor such as WWOX and positively regulating pro-survival factors such as AKT1 and AR, Contributes to the control of AXL receptor levels, Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1, Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin, MCF2, May be involved both in adult synaptic function and plasticity and in brain development, Phosphorylates AKT1, Phosphorylates AR on 'Tyr-267' and 'Tyr-363' thereby promoting its recruitment to androgen-responsive enhancers (AREs), Phosphorylates MCF2, Phosphorylates WWOX on 'Tyr-287', Transduces extracellular signals to cytosolic and nuclear effectors, WASL and WWOX, cell growth and proliferation, cell survival, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes, thereby enhancing its activity as a guanine nucleotide exchange factor (GEF) toward Rho family proteins, Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
p21cdc42Hs ACK ACK1 |
| Also known as: |
Ack1 Polyclonal Antibody |
| Other name: |
Anti-Ack1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Ack1 |
| Short name: |
Anti-Ack1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Ack1 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
19297 |
| Gene: |
TNK2 |
More about : TNK2 |
| Long gene name: |
tyrosine kinase non receptor 2 |
| Synonyms gene name: |
2 , non-receptor, tyrosine kinase |
| Synonyms name: |
activated Cdc42-associated kinase 1 |
| Locus: |
3q29 |
| Discovery year: |
2005-01-19 |
| GenBank acession: |
L13738 |
| Entrez gene record: |
10188 |
| Pubmed identfication: |
8497321 14506255 |
| RefSeq identity: |
NM_005781 |
| Havana BLAST/BLAT: |
OTTHUMG00000155737 |