| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
MELK/HPK38 |
| Specificity: |
This antibody reacts specifically with MELK/HPK38 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cell membrane |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human MELK/HPK38 |
| Gene ID: |
9833 |
| Swiss Prot: |
Q14680 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Acts as a regulator of cell cycle, Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1, Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, CDC25B, Has a broad substrate specificity, MAP3K5/ASK1 and ZNF622, May also play a role in primitive hematopoiesis, Phosphorylates and inhibits BCL2L14, Plays a key role in cell proliferation and carcinogenesis, Required for proliferation of embryonic and postnatal multipotent neural progenitors, apoptosis and splicing regulation, notably by mediating phosphorylation of CDC25B, phosphorylates BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis, self-renewal of stem cells, thereby contributing to its redirection to the nucleus, Serine/threonine-protein kinase involved in various processes such as cell cycle regulation |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
KIAA0175 |
| Also known as: |
MELK/HPK38 Polyclonal Antibody |
| Other name: |
Anti-MELK/HPK38 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
MELK/HPK38 |
| Short name: |
Anti-MELK/HPK38 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-MELK/HPK38 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
16870 |
| Gene: |
MELK |
More about : MELK |
| Long gene name: |
maternal embryonic leucine zipper kinase |
| Locus: |
9p13, 2 |
| Discovery year: |
2003-02-04 |
| GenBank acession: |
D79997 |
| Entrez gene record: |
9833 |
| Pubmed identfication: |
8724849 9136115 |
| RefSeq identity: |
NM_014791 |
| Havana BLAST/BLAT: |
OTTHUMG00000019906 |