| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
NCS1/Frequenin |
| Specificity: |
This antibody reacts specifically with NCS1/Frequenin |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human NCS1 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
It is also expressed in glial cells and in neuroendocrine bovine adrenal chromaffin and PC12 cells, NCS-1 also functions in the voltage-independent autocrine pathway that negatively regulates non-L-type Ca++ channels, NCS-1 is a regulatory protein involved in Ca++-dependent exocytosis of synaptic vesicles and dense core granules, NCS-1 is localized to neuronal cell bodies and axons throughout the brain and spinal cord, also designated frequenin) belongs to a highly conserved family of EF-hand-containing Ca++-binding proteins expressed mainly in neurons, NCS-1 (for neuronal calcium sensor-1 |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
NCS-1 |
| Also known as: |
NCS1/Frequenin Polyclonal Antibody |
| Other name: |
Anti-NCS1/Frequenin Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
NCS1/Frequenin |
| Short name: |
Anti-NCS1/Frequenin (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-neuronal calcium sensor 1/Frequenin (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
BT, Cell surfaces, FLUP and FREQ, NCS1 and IDBG-89459 and ENSG00000107130 and 23413, Ncs1 and IDBG-157707 and ENSMUSG00000062661 and 14299, protein kinase binding, this GO :0000287 and magnesium ion binding and molecular function this GO :0005245 and voltage-gated calcium channel activity and molecular function this GO :0005509 and calcium ion binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0010975 and regulation of neuron projection development and biological process this GO :0014069 and postsynaptic density and cellular component this GO :0019901 and protein kinase binding and molecular function this GO :0030054 and cell junction and cellular component this GO :0030424 and axon and cellular component this GO :0030425 and dendrite and cellular component this GO :0031045 and dense core granule and cellular component this GO :0031410 and cytoplasmic vesicle and cellular component this GO :0032580 and this GO lgi cisterna membrane and cellular component this GO :0043231 and intracellular membrane-bounded organelle and cellular component this GO :0045211 and postsynaptic membrane and cellular component this GO :0045921 and positive regulation of exocytosis and biological process this GO :0048015 and phosphatidylinositol-mediated signaling and biological process this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0070588 and calcium ion transmembrane transport and biological process, this GO :0000287 : magnesium ion binding, this GO :0000287 : magnesium ion binding and also this GO :0005245 : voltage-gated calcium channel activity and also this GO :0005509 : calcium ion binding and also this GO :0005515 : protein binding and also this GO :0019901 : protein kinase binding, this GO :0005245 : voltage-gated calcium channel activity, this GO :0005509 : calcium ion binding, this GO :0005515 : protein binding, this GO :0019901 : protein kinase binding, 40187 and IDBG-639872 and ENSBTAG00000008726 and 526544, neuronal calcium sensor 1 |
| Identity: |
3953 |
| Gene: |
NCS1 |
More about : NCS1 |
| Long gene name: |
neuronal calcium sensor 1 |
| Synonyms gene: |
FREQ |
| Synonyms gene name: |
frequenin (Drosophila) homolog frequenin homolog (Drosophila) |
| Locus: |
9q34, 11 |
| Discovery year: |
2000-06-09 |
| GenBank acession: |
AF186409 |
| Entrez gene record: |
23413 |
| Pubmed identfication: |
11092894 26705828 |
| RefSeq identity: |
NM_014286 |
| Classification: |
EF-hand domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000020801 |