| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
LIM kinase 1 + 2 |
| Specificity: |
This antibody reacts specifically with LIM kinase 1 + 2 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human LIM kinase 1 + 2 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
LIMK1 is highly active in the brain and spinal chord, LIMK1 is specifically stimulated by Rac, LIMK1 may play an important role in areas of the brain that are responsible for processing visual-spatial information (visuospatial constructive cognition), Rho and Cdc42, These parts of the brain are important for visualizing an object as a set of parts and performing tasks such as writing, They can phosphorylate an actin-binding protein, and assembling puzzles, cofilin which binds to actin monomers and polymers and promotes the disassembly of actin filament, constructing models, drawing, one of the Rho family proteins, suggesting that two distinct pathways exist in the Rho family driven actin cytoskeleton dynamics, through control of the organization of actin filaments, where it is believed to be involved in the development of nerve cells whilst LIMK2 is ubiquitously expressed in many adult tissues, while LIMK2 activity is activated under the control of other Rho family members, LIMK 1 and 2 likely regulate aspects of the cytoskeleton, The phosphorylation of cofilin via LIMK inactivates this potential |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
LIM Enh |
| Also known as: |
LIM kinase 1 + 2 Polyclonal Antibody |
| Other name: |
Anti-LIM kinase 1 + 2 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
LIM kinase 1 + 2 |
| Short name: |
Anti-LIM kinase 1 + 2 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-LIM phosphorylation catalyst 1 and 2 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
17468 |
| Gene: |
PDLIM5 |
More about : PDLIM5 |
| Long gene name: |
PDZ and LIM domain 5 |
| Locus: |
4q22, 3 |
| Discovery year: |
2005-01-12 |
| GenBank acession: |
AF061258 |
| Entrez gene record: |
10611 |
| Pubmed identfication: |
15346770 |
| Classification: |
LIM domain containing PDZ domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000130973 |