| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Gbx2 |
| Specificity: |
This antibody reacts specifically with Gbx2 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Gbx2 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
DU145 and LNCaP), During development, During development, Furthermore, Gbx2 and Otx2 play distinct, Gbx2 is expressed in the adult brain, Gbx2 negatively regulates Otx2 expression along the anterior-posterior axis, Gbx2(-) mutants demonstrate an expanded Otx2 domain, PC3, Specifically, The GBX2 gene is over-expressed in human prostate cancer cell lines (TSU-prl, The MHB forms at the boundary of expression between homeobox genes Gbx2 and Otx2, downregulation of Gbx2 expression restricts tumorigenicity in human prostate cancer cell lines, essential roles in MHB positioning and development, spleen and female genital tract, the GBX2 gene is expressed in the anterior hindbrain, the GBX2 gene is expressed in the anterior hindbrain, which suggests that Gbx2 expression may be required for growth of malignant prostate cells, The isthmic organizer signals at the mid/hindbrain boundary (MHB) regulate the development and differentiation of the vertebrate caudal midbrain and the anterior hindbrain |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
Gbx2 Polyclonal Antibody |
| Other name: |
Anti-Gbx2 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Gbx2 |
| Short name: |
Anti-Gbx2 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-gastrulation brain homeobox 2 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
4186 |
| Gene: |
GBX2 |
More about : GBX2 |
| Long gene name: |
gastrulation brain homeobox 2 |
| Synonyms gene name: |
gastrulation brain homeo box 2 |
| Locus: |
2q37, 2 |
| Discovery year: |
1997-10-30 |
| GenBank acession: |
AF118452 |
| Entrez gene record: |
2637 |
| Pubmed identfication: |
9346236 8838315 |
| RefSeq identity: |
NM_001485 |
| Classification: |
HOXL subclass homeoboxes |
| Havana BLAST/BLAT: |
OTTHUMG00000133294 |