| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
GRK3/Adrbk2 |
| Specificity: |
This antibody reacts specifically with GRK3/Adrbk2 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Adrbk2 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
At least seven members of the GRK family have been identified, Beta ARK1) and GRK 3 (Beta ARK2), GRK 5, GRK 6 and GRK 7, IT-11 (GRK 4), Phosphorylation of receptors by GRKs appears to be strictly dependent on the receptor being in its agonist-activated state, The first class is comprised of the second messenger-regulated kinases, The second class includes the G protein-coupled receptor kinases (GRKs), These include rhodopsin kinase (GRK 1), This phenomenon, involves phosphorylation of the receptor by two classes of enzymes, referred to as agonist-mediated desensitization, such as c-AMP dependent protein kinase A and protein kinase C, two forms of Beta-adrenergic receptor kinase: GRK 2 (Beta ARK, Heterotrimeric G protein-mediated signal transduction is a dynamically regulated process with the intensity of signal decreasing over time despite the continued presence of the agonist |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
BARK2 |
| Also known as: |
GRK3/Adrbk2 Polyclonal Antibody |
| Other name: |
Anti-GRK3/Adrbk2 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
GRK3/Adrbk2 |
| Short name: |
Anti-GRK3/Adrbk2 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-GRK3/Adrbk2 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
290 |
| Gene: |
GRK3 |
More about : GRK3 |
| Long gene name: |
G protein-coupled receptor kinase 3 |
| Synonyms gene: |
ADRBK2 |
| Synonyms gene name: |
adrenergic, beta, receptor kinase 2 |
| Locus: |
22q12, 1 |
| Discovery year: |
1992-03-25 |
| GenBank acession: |
X69117 |
| Entrez gene record: |
157 |
| Pubmed identfication: |
7695743 |
| RefSeq identity: |
NM_005160 |
| Classification: |
Pleckstrin homology domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000150280 |