| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
UCHL3 |
| Specificity: |
This antibody reacts specifically with UCHL3 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human UCHL3 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Examination of specific brain regions reveals expression in all areas tested, Expression of UCH-L1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors, Its occurrence in Lewy bodies and its function in the proteasome pathway make it a compelling candidate gene in Parkinson disease, The 230 amino acid human UCH-L3 protein is 54% identical to that of UCH-L1, The gene which encodes UCH-L1 maps to human chromosome 4p14, UCH-L1 is expressed in brain neurons, UCH-L1 represents 1 to 2% of total soluble brain protein, UCH-L3 is the predominant thiol protease and has high-affinity binding sites for ubiquitin, particularly in the substantia nigra, UCH-L1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
UCHL3 Polyclonal Antibody |
| Other name: |
Anti-UCHL3 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
UCHL3 |
| Short name: |
Anti-UCHL3 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-UCHL3 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
12515 |
| Gene: |
UCHL3 |
More about : UCHL3 |
| Long gene name: |
ubiquitin C-terminal hydrolase L3 |
| Synonyms gene name: |
ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) |
| Synonyms name: |
ubiquitin thiolesterase |
| Locus: |
13q22, 2 |
| Discovery year: |
1991-07-15 |
| GenBank acession: |
M30496 |
| Entrez gene record: |
7347 |
| Pubmed identfication: |
2530630 |
| RefSeq identity: |
NM_006002 |
| Classification: |
Ubiquitin C-terminal hydrolases |
| Havana BLAST/BLAT: |
OTTHUMG00000017090 |