| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Piccolo |
| Specificity: |
This antibody reacts specifically with Piccolo |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Piccolo |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Bassoon, Bassoon is a large protein which is also found in the presynaptic compartments of rat brain synapses, Bassoon is found in axon terminals of hippocampal neurons where it is highly concentrated in the vicinity of the active zone, Bassoon may be involved in cytomatrix organization at the site of neurotransmitter release, Piccolo has a similar distribution and colocalizes with Bassoon in cultured hippocampal cells, Piccolo zinc fingers interact with the dual prenylated rab3A and VAMP2/ Synaptobrevin II receptor PRA, The gene which encodes bassoon maps to human chromosome 3p21, The gene which encodes piccolo maps to human chromosome 7q21, along with Piccolo, is part of the ensemble of presynaptic proteins that are involved in orchestrating events at the nerve terminal, 11, 31, Piccolo is 1 component of the presynaptic cytomatrix |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
KIAA0559 DKFZp779G1236 ACZ |
| Also known as: |
Piccolo Polyclonal Antibody |
| Other name: |
Anti-Piccolo Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Piccolo |
| Short name: |
Anti-Piccolo (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Piccolo (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
13406 |
| Gene: |
PCLO |
More about : PCLO |
| Long gene name: |
piccolo presynaptic cytomatrix protein |
| Synonyms gene name: |
piccolo (presynaptic cytomatrix protein) |
| Synonyms name: |
aczonin |
| Locus: |
7q21, 11 |
| Discovery year: |
2001-06-25 |
| GenBank acession: |
AB011131 |
| Entrez gene record: |
27445 |
| Pubmed identfication: |
8900486 9628581 |
| RefSeq identity: |
NM_014510 |
| Classification: |
Zinc fingers PDZ domain containing C2 domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000154853 |