| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Islet 2 |
| Specificity: |
This antibody reacts specifically with Islet 2 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Islet 2 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
An early phase of Islet-2 expression by prospective visceral motor neurons of the sympathetic preganglionic motor column is critical for the emergence of complete visceral motor neuron character, Islet-1 and Islet-2 are initially ex-pressed by all postmitotic spinal motor neurons prior to diversification of somatic and visceral neuronal fates, Islet-2 is a nuclear protein that contains two N-terminal LIM domains, Islet-2 is a transcriptional factor that defines subclasses of motor neurons that segregate into columns in the spinal cord and select distinct axon pathways, Mutations that reduce or eliminate both Islet-1 and Islet-2 activity will result in pronounced defects in visceral motor neuron generation and eroded somatic motor neuron character, Somatic, but not visceral, followed by a homeodomain and a serine/ glutamine/threonine-rich C-terminus, motor neurons maintain Islet-2 expression at later embryonic stages, Islet-2 (insulin gene enhancer protein ISL-2) is a 359 amino acid protein encoded by the human gene ISL2 |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
Islet 2 Polyclonal Antibody |
| Other name: |
Anti-Islet 2 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
Islet 2 |
| Short name: |
Anti-Islet 2 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-Islet 2 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
14442 |
| Gene: |
ICA1L |
More about : ICA1L |
| Long gene name: |
islet cell autoantigen 1 like |
| Synonyms gene: |
ALS2CR15 ALS2CR14 |
| Synonyms gene name: |
amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 14 islet cell autoantigen 1, candidate 15 amyotrophic lateral sclerosis 2 (juvenile) chromosome region, 69kDa-like |
| Locus: |
2q33, 2 |
| Discovery year: |
2001-01-26 |
| GenBank acession: |
AB053317 |
| Entrez gene record: |
130026 |
| Pubmed identfication: |
11586298 |
| RefSeq identity: |
NM_138468 |
| Classification: |
Classical BAR domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000132856 |