| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
TCF12/HEB |
| Specificity: |
This antibody reacts specifically with TCF12/HEB |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human TCF12 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Both homo- and hetero-oligomers of these proteins are able to distinguish very closely related E box proteins and are believed to play important roles in lineage specific gene expression, IF2 and HEB, Myf-5 and Myf-6 (also designated MRF-4 or herculin), Myo D transcription factors form heterodimers with products of a more widely expressed family of bHLH genes, Myo D-E heterodimers bind avidly to consensus (CANNTG) E box target sites that are functionally important elements in the upstream regulatory sequences of many muscle-specific terminal differentiation genes, One well characterized family of helix-loop-helix (HLH) proteins known to play an important role in the regulation of muscle cell development includes Myo D, myogenin, the E family, which consists of at least three distinct genes: E2A, Differentiation of myogenic cells is regulated by multiple positively and negatively acting factors |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
HEB HTF4 HsT17266 bHLHb20 |
| Also known as: |
TCF12/HEB Polyclonal Antibody |
| Other name: |
Anti-TCF12/HEB Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
TCF12/HEB |
| Short name: |
Anti-TCF12/HEB (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-TCF12/HEB (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
11623 |
| Gene: |
TCF12 |
More about : TCF12 |
| Long gene name: |
transcription factor 12 |
| Synonyms name: |
helix-loop-helix transcription factor 4 |
| Locus: |
15q21, 3 |
| Discovery year: |
1994-06-17 |
| GenBank acession: |
BC050556 |
| Entrez gene record: |
6938 |
| Pubmed identfication: |
1886779 |
| RefSeq identity: |
NM_003205 |
| Classification: |
Basic helix-loop-helix proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000132047 |