| Antigen detectable with this ELISA kit: |
Cadherin 5 (CDH5) |
| Species reactivity: |
Mus musculus (Mouse) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
059ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cadherin 5 (CDH5), The concentration of Cadherin 5 (CDH5) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cadherin 5 (CDH5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Cadherin 5 (CDH5), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Cadherin 5 (CDH5) |
| Short name: |
Cadherin 5 (CDH5) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
type 2 (vascular endothelium)) Enzyme-linked immunosorbent assay reagent, Cadherin 5 (cadherin 5 |
| Alternative technique: |
elisas |
| Alternative to gene target: |
7B4 and CD144, BT, CDH5 and IDBG-34871 and ENSG00000179776 and 1003, Cdh5 and IDBG-185038 and ENSMUSG00000031871 and 12562, Cell surfaces, ion channel binding, this GO :0001955 and blood vessel maturation and biological process this GO :0005102 and receptor binding and molecular function this GO :0005509 and calcium ion binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005886 and plasma membrane and cellular component this GO :0005911 and cell-cell junction and cellular component this GO :0005923 and tight junction and cellular component this GO :0007156 and homophilic cell adhesion and biological process this GO :0008013 and beta-catenin binding and molecular function this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0016020 and membrane and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0019903 and protein phosphatase binding and molecular function this GO :0030054 and cell junction and cellular component this GO :0034329 and cell junction assembly and biological process this GO :0034332 and adherens junction organization and biological process this GO :0044325 and ion channel binding and molecular function this GO :0045216 and cell-cell junction organization and biological process this GO :0071944 and cell periphery and cellular component this GO :2000114 and regulation of establishment of cell polarity and biological process, this GO :0005102 : receptor binding, this GO :0005102 : receptor binding and also this GO :0005509 : calcium ion binding and also this GO :0005515 : protein binding and also this GO :0008013 : beta-catenin binding and also this GO :0019903 : protein phosphatase binding and also this GO :0044325 : ion channel binding, this GO :0005509 : calcium ion binding, this GO :0005515 : protein binding, this GO :0008013 : beta-catenin binding, this GO :0019903 : protein phosphatase binding, this GO :0044325 : ion channel binding, type 2 (vascular endothelium), 64659 and IDBG-633039 and ENSBTAG00000007421 and 414735, cadherin 5 |
| Identity: |
1764 |
| Gene: |
CDH5 |
More about : CDH5 |
| Long gene name: |
cadherin 5 |
| Synonyms gene name: |
VE-cadherin (vascular epithelium) cadherin 5, cadherin 5, type 2, type 2 (vascular endothelium) |
| Synonyms: |
7B4 CD144 |
| Synonyms name: |
VE-cadherin |
| Locus: |
16q21 |
| Discovery year: |
1992-11-20 |
| GenBank acession: |
X79981 |
| Entrez gene record: |
1003 |
| Pubmed identfication: |
2059658 |
| RefSeq identity: |
NM_001795 |
| Classification: |
Type II classical cadherins CD molecules |
| Havana BLAST/BLAT: |
OTTHUMG00000137495 |