| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
NTN3/Netrin-3 |
| Specificity: |
This antibody reacts specifically with NTN3/Netrin-3 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human NTN3/Netrin-3 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
During peripheral nerve development, In axonal cells, In humans, Neogenin serves as the primary guidance receptor for netrin-3, Neogenin serves as the primary guidance receptor for netrin-3, Netrin signaling is dependent on the concentration of calcium outside the cell and the level of PKA activity, Netrin-2 and the corresponding mouse homolog netrin-3 are expressed primarily in the lower two-thirds of the spinal cord, Netrin-3 proteins are associated with the axon fibers projecting from motor neurons and from neurons within sympathetic and sensory ganglia, a reduction in PKA activity converts the responsiveness of the axons to the netrin proteins as the cells are repelled, and, by the netrin gradient, high netrin-3 expression has been detected in mesenchymal cells, like netrin-1, rather than attracted, sensory ganglia and muscles, suggesting that netrin-3 may be involved in pathfinding and fasciculation of axon projection, the gene encoding for the netrin-3 protein is localized to chromosome 16p13, they can either attract or repel commissural axons at a distance, 3, Netrin proteins are a family of laminin-related secreted proteins that provide guidance signals for axonal growth and cell migration during development |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Also known as: |
NTN3/Netrin-3 Polyclonal Antibody |
| Other name: |
Anti-NTN3/Netrin-3 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
NTN3/Netrin-3 |
| Short name: |
Anti-NTN3/Netrin-3 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-NTN3/Netrin-3 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
8030 |
| Gene: |
NTN3 |
More about : NTN3 |
| Long gene name: |
netrin 3 |
| Synonyms gene: |
NTN2L |
| Synonyms gene name: |
netrin 2 (chicken)-like netrin 2-like (chicken) |
| Synonyms name: |
Netrin-3 |
| Locus: |
16p13, 3 |
| Discovery year: |
1997-02-05 |
| GenBank acession: |
U86759 |
| Entrez gene record: |
4917 |
| Pubmed identfication: |
9143507 10366627 |
| RefSeq identity: |
NM_006181 |
| Classification: |
Netrins |
| Havana BLAST/BLAT: |
OTTHUMG00000128867 |