| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
ITCH / AIF4 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
1-0, 5ug/ml, Western blot: 0 |
| Notes: |
Optimal dilution of the ITCH antibody should be determined by the researcher |
| Intented use: |
This ITCH antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q96J02 |
| Purity: |
Antigen affinity |
| Description: |
Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, Mutations in this gene are a cause of syndromic multisystem autoimmune disease, The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses, This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases, thus targeting specific proteins for lysosomal degradation, ITCH is an ubiquitin-conjugating enzyme |
| Immunogen: |
Amino acids AMQQFNQRFIYGNQDLFATSQSKEFDPL of human ITCH were used as the immunogen for the ITCH antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the ITCH antibody may be kept for up to one month refrigerated at +4 degrees C, After reconstitution, For long-term |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
ITCH / AIF4 |
| Short name: |
ITCH Antibody / AIF4 |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
itchy E3 ubiquitin protein ligase (Antibody to) / AIF4 |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
CXCR chemokine receptor binding, ITCH and IDBG-641890 and ENSBTAG00000000308 and 508431, ITCH and IDBG-68143 and ENSG00000078747 and 83737, Itch and IDBG-210517 and ENSMUSG00000027598 and 16396, leucine rich repeat containing receptor signaling pathway and biological process this GO :0042787 and protein ubiquitination involved in ubiquitin-dependent protein catabolic process and biological process this GO :0043021 and ribonucleoprotein complex binding and molecular function this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0045087 and innate immune response and biological process this GO :0045236 and CXCR chemokine receptor binding and molecular function this GO :0045732 and positive regulation of protein catabolic process and biological process this GO :0046329 and negative regulation of JNK cascade and biological process this GO :0046642 and negative regulation of alpha-beta T cell proliferation and biological process this GO :0046718 and viral entry into host cell and biological process this GO :0050687 and negative regulation of defense response to virus and biological process this GO :0051607 and defense response to virus and biological process this GO :0070423 and nucleotide-binding oli this GO merization domain containing signaling pathway and biological process this GO :0070534 and protein K63-linked ubiquitination and biological process this GO :0070936 and protein K48-linked ubiquitination and biological process this GO :0090085 and regulation of protein deubiquitination and biological process, nuclei, this GO :0000209 and protein polyubiquitination and biological process this GO :0001558 and regulation of cell growth and biological process this GO :0002669 and positive regulation of T cell anergy and biological process this GO :0004842 and ubiquitin-protein transferase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005938 and cell cortex and cellular component this GO :0006511 and ubiquitin-dependent protein catabolic process and biological process this GO :0006915 and apoptotic process and biological process this GO :0006954 and inflammatory response and biological process this GO :0007219 and Notch signaling pathway and biological process this GO :0016020 and membrane and cellular component this GO :0016567 and protein ubiquitination and biological process this GO :0016874 and ligase activity and molecular function this GO :0031410 and cytoplasmic vesicle and cellular component this GO :0032088 and negative regulation of NF-kappaB transcription factor activity and biological process this GO :0032480 and negative regulation of type I interferon production and biological process this GO :0035519 and protein K29-linked ubiquitination and biological process this GO :0035872 and nucleotide-binding domain, this GO :0004842 : ubiquitin-protein transferase activity, this GO :0004842 : ubiquitin-protein transferase activity and also this GO :0005515 : protein binding and also this GO :0016874 : ligase activity and also this GO :0043021 : ribonucleoprotein complex binding and also this GO :0045236 : CXCR chemokine receptor binding, this GO :0005515 : protein binding, this GO :0016874 : ligase activity, this GO :0043021 : ribonucleoprotein complex binding, this GO :0045236 : CXCR chemokine receptor binding, itchy E3 ubiquitin protein ligase |
| Identity: |
13890 |
| Gene: |
ITCH |
More about : ITCH |
| Long gene name: |
itchy E3 ubiquitin protein ligase |
| Synonyms gene name: |
itchy (mouse homolog) E3 ubiquitin protein ligase itchy E3 ubiquitin protein ligase homolog (mouse) |
| Synonyms: |
AIP4 |
| Locus: |
20q11, 22 |
| Discovery year: |
2001-04-27 |
| GenBank acession: |
AF095745 |
| Entrez gene record: |
83737 |
| Pubmed identfication: |
11318614 |
| Classification: |
C2 domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000032300 |
| Locus Specific Databases: |
LRG_354 |