| Antigen detectable with this ELISA kit: |
Beclin 1 (BECN1) |
| Species reactivity: |
Rattus norvegicus (Rat) |
| Range of detection: |
0, 0, 0, 1, 2, 5ng/mL, 156-10ng/mL The standard curve concentrations used for the ELISA&rsquo, 156ng/mL, 25ng/mL, 313ng/mL, 5ng/mL, 625ng/mL, s were 10ng/mL |
| Sensitivity: |
058ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, plasma, tissue homogenates, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Beclin 1 (BECN1), The concentration of Beclin 1 (BECN1) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beclin 1 (BECN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Beclin 1 (BECN1), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Beclin 1 (BECN1) |
| Short name: |
Beclin 1 (BECN1) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
autophagy related) Enzyme-linked immunosorbent assay reagent, Beclin 1 (beclin 1 |
| Alternative technique: |
elisas |
| Alternative to gene target: |
ATG6 and beclin1 and VPS30, BECN1 and IDBG-51769 and ENSG00000126581 and 102725513, BECN1 and IDBG-640456 and ENSBTAG00000019914 and 527278, Becn1 and IDBG-212053 and ENSMUSG00000035086 and 56208, Plasma membranes, autophagy related, sequence-specific DNA binding, this GO :0000910 and cytokinesis and biological process this GO :0005515 and protein binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005802 and trans- this GO lgi network and cellular component this GO :0006914 and autophagy and biological process this GO :0006968 and cellular defense response and biological process this GO :0007040 and lysosome organization and biological process this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0016020 and membrane and cellular component this GO :0016023 and cytoplasmic membrane-bounded vesicle and cellular component this GO :0016032 and viral process and biological process this GO :0016239 and positive regulation of macroautophagy and biological process this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043234 and protein complex and cellular component this GO :0043565 and sequence-specific DNA binding and molecular function this GO :0045087 and innate immune response and biological process this GO :0045335 and pha this GO cytic vesicle and cellular component this GO :0048666 and neuron development and biological process this GO :0050435 and beta-amyloid metabolic process and biological process this GO :0050790 and regulation of catalytic activity and biological process this GO :0051607 and defense response to virus and biological process this GO :0051707 and response to other organism and biological process, this GO :0005515 : protein binding, this GO :0005515 : protein binding and also this GO :0043565 : sequence-specific DNA binding, this GO :0043565 : sequence-specific DNA binding, 8678, beclin 1 |
| Identity: |
1034 |
| Gene: |
BECN1 |
More about : BECN1 |
| Long gene name: |
beclin 1 |
| Synonyms gene name: |
autophagy related , beclin 1 (coiled-coil, moesin-like BCL2 interacting protein) beclin 1 |
| Synonyms: |
ATG6 VPS30 |
| Synonyms name: |
ATG6 autophagy related 6 homolog (S, cerevisiae) |
| Locus: |
17q21, 31 |
| Discovery year: |
1998-11-27 |
| GenBank acession: |
AF077301 |
| Entrez gene record: |
8678 |
| Pubmed identfication: |
9765397 |
| RefSeq identity: |
NM_003766 |
| Classification: |
Autophagy related |
| Havana BLAST/BLAT: |
OTTHUMG00000180653 |