| Antigen detectable with this ELISA kit: |
Bcl2 Antagonist/Killer 1 (BAK1) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 1, 10ng/mL, 2, 20ng/mL, 5ng/mL, 25ng/mL, 5ng/mL, 625-40ng/mL The standard curve concentrations used for the ELISA&rsquo, 625ng/mL, s were 40ng/mL |
| Sensitivity: |
217ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell lysates and other biological fluids, Tissue homogenates |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bcl2 Antagonist/Killer 1 (BAK1), The concentration of Bcl2 Antagonist/Killer 1 (BAK1) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bcl2 Antagonist/Killer 1 (BAK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Bcl2 Antagonist/Killer 1 (BAK1), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Bcl2 Antagonist/Killer 1 (BAK1) |
| Short name: |
Bcl2 Antagonist/Killer 1 (BAK1) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
B-cell CLL/lymphoma 2 Antagonist/Killer 1 (B-cell CLL/lymphoma 2-anta this GO nist/killer 1) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
BAK and BAK-LIKE and BCL2L7 and CDN1, BAK1 and IDBG-629109 and ENSBTAG00000000428 and 514090, BAK1 and IDBG-82944 and ENSG00000030110 and 578, BH domain binding, Bak1 and IDBG-156207 and ENSMUSG00000057789 and 12018, Plasma membranes, this GO :0001776 and leukocyte homeostasis and biological process this GO :0001782 and B cell homeostasis and biological process this GO :0001783 and B cell apoptotic process and biological process this GO :0001836 and release of cytochrome c from mitochondria and biological process this GO :0001974 and blood vessel remodeling and biological process this GO :0002262 and myeloid cell homeostasis and biological process this GO :0002352 and B cell negative selection and biological process this GO :0005515 and protein binding and molecular function this GO :0005622 and intracellular and cellular component this GO :0005739 and mitochondrion and cellular component this GO :0005741 and mitochondrial outer membrane and cellular component this GO :0005783 and endoplasmic reticulum and cellular component this GO :0005829 and cytosol and cellular component this GO :0006915 and apoptotic process and biological process this GO :0006919 and activation of cysteine-type endopeptidase activity involved in apoptotic process and biological process this GO :0007420 and brain development and biological process this GO :0007568 and aging and biological process this GO :0008053 and mitochondrial fusion and biological process this GO :0008283 and cell proliferation and biological process this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0008630 and intrinsic apoptotic signaling pathway in response to DNA damage and biological process this GO :0008635 and activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c and biological process this GO :0009620 and response to fungus and biological process this GO :0010046 and response to mycotoxin and biological process this GO :0010225 and response to UV-C and biological process this GO :0010248 and establishment or maintenance of transmembrane electrochemical gradient and biological process this GO :0010332 and response to gamma radiation and biological process this GO :0010524 and positive regulation of calcium ion transport into cytosol and biological process this GO :0010629 and negative regulation of gene expression and biological process this GO :0014070 and response to organic cyclic compound and biological process this GO :0016020 and membrane and cellular component this GO :0031018 and endocrine pancreas development and biological process this GO :0031072 and heat shock protein binding and molecular function this GO :0031100 and organ regeneration and biological process this GO :0031307 and integral component of mitochondrial outer membrane and cellular component this GO :0031966 and mitochondrial membrane and cellular component this GO :0032469 and endoplasmic reticulum calcium ion homeostasis and biological process this GO :0032471 and negative regulation of endoplasmic reticulum calcium ion concentration and biological process this GO :0033137 and negative regulation of peptidyl-serine phosphorylation and biological process this GO :0034644 and cellular response to UV and biological process this GO :0035108 and limb morphogenesis and biological process this GO :0042493 and response to drug and biological process this GO :0042542 and response to hydrogen peroxide and biological process this GO :0042802 and identical protein binding and molecular function this GO :0042803 and protein homodimerization activity and molecular function this GO :0042981 and regulation of apoptotic process and biological process this GO :0043065 and positive regulation of apoptotic process and biological process this GO :0043496 and regulation of protein homodimerization activity and biological process this GO :0043497 and regulation of protein heterodimerization activity and biological process this GO :0044346 and fibroblast apoptotic process and biological process this GO :0045471 and response to ethanol and biological process this GO :0045862 and positive regulation of proteolysis and biological process this GO :0046872 and metal ion binding and molecular function this GO :0046902 and regulation of mitochondrial membrane permeability and biological process this GO :0046930 and pore complex and cellular component this GO :0046982 and protein heterodimerization activity and molecular function this GO :0048597 and post-embryonic camera-type eye morphogenesis and biological process this GO :0048872 and homeostasis of number of cells and biological process this GO :0051087 and chaperone binding and molecular function this GO :0051400 and BH domain binding and molecular function this GO :0051726 and regulation of cell cycle and biological process this GO :0051881 and regulation of mitochondrial membrane potential and biological process this GO :0060068 and vagina development and biological process this GO :0070059 and intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress and biological process this GO :0070242 and thymocyte apoptotic process and biological process this GO :0071260 and cellular response to mechanical stimulus and biological process this GO :0090200 and positive regulation of release of cytochrome c from mitochondria and biological process this GO :0097190 and apoptotic signaling pathway and biological process this GO :0097192 and extrinsic apoptotic signaling pathway in absence of ligand and biological process this GO :0097193 and intrinsic apoptotic signaling pathway and biological process this GO :0097202 and activation of cysteine-type endopeptidase activity and biological process this GO :1900103 and positive regulation of endoplasmic reticulum unfolded protein response and biological process this GO :1901030 and positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway and biological process this GO :1902262 and apoptotic process involved in patterning of blood vessels and biological process, this GO :0005515 : protein binding, this GO :0005515 : protein binding and also this GO :0031072 : heat shock protein binding and also this GO :0042802 : identical protein binding and also this GO :0042803 : protein homodimerization activity and also this GO :0046872 : metal ion binding and also this GO :0046982 : protein heterodimerization activity and also this GO :0051087 : chaperone binding and also this GO :0051400 : BH domain binding, this GO :0031072 : heat shock protein binding, this GO :0042802 : identical protein binding, this GO :0042803 : protein homodimerization activity, this GO :0046872 : metal ion binding, this GO :0046982 : protein heterodimerization activity, this GO :0051087 : chaperone binding, this GO :0051400 : BH domain binding, BCL2-anta this GO nist/killer 1 |
| Identity: |
949 |
| Gene: |
BAK1 |
More about : BAK1 |
| Long gene name: |
BCL2 antagonist/killer 1 |
| Synonyms gene: |
CDN1 |
| Synonyms gene name: |
BCL2-antagonist/killer 1 |
| Synonyms: |
BCL2L7 BAK |
| Locus: |
6p21, 31 |
| Discovery year: |
1997-10-09 |
| GenBank acession: |
U23765 |
| Entrez gene record: |
578 |
| Pubmed identfication: |
7715730 7715731 |
| RefSeq identity: |
NM_001188 |
| Classification: |
BCL2 family |
| Havana BLAST/BLAT: |
OTTHUMG00000014530 |